9ojj: Difference between revisions
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New page: '''Unreleased structure''' The entry 9ojj is ON HOLD Authors: White, K.I., Brunger, A.T. Description: sx20S complex (NSF-alphaSNAP-syntaxin-1a), alphaSNAP-syntaxin-1a subcomplex local ... |
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==sx20S complex (NSF-alphaSNAP-syntaxin-1a), 4:4 alphaSNAP-syntaxin-1a subcomplex local refinement, non-hydrolyzing, class 1== | |||
<StructureSection load='9ojj' size='340' side='right'caption='[[9ojj]], [[Resolution|resolution]] 4.07Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9ojj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9OJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9OJJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.07Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ojj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ojj OCA], [https://pdbe.org/9ojj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ojj RCSB], [https://www.ebi.ac.uk/pdbsum/9ojj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ojj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Synaptic vesicles containing neurotransmitters fuse with the plasma membrane upon the arrival of an action potential at the active zone. Multiple proteins organize trans-SNARE complex assembly and priming, leading to fusion. One target membrane SNARE, syntaxin, forms nanodomains at the active zone, and another, SNAP-25, enters non-fusogenic complexes with it. Here, we reveal mechanistic details of AAA+ protein NSF (N-ethylmaleimide sensitive factor) and SNAP (soluble NSF attachment protein) action before fusion. We show that syntaxin clusters are conserved, that NSF colocalizes with them, and characterize SNARE populations that may exist within or near them using cryo-EM. Supercomplexes of NSF, alpha-SNAP, and either a syntaxin tetramer or one of two binary complexes of syntaxin-SNAP-25 reveal atomic details of SNARE processing and show how sequential ATP hydrolysis drives disassembly. These results suggest a functional role for syntaxin clusters as reservoirs and a corresponding role for NSF in syntaxin liberation and SNARE protein quality control preceding fusion. | |||
Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission.,White KI, Khan YA, Qiu K, Balaji A, Couoh-Cardel S, Esquivies L, Pfuetzner RA, Diao J, Brunger AT Nat Commun. 2025 Sep 24;16(1):8371. doi: 10.1038/s41467-025-62764-0. PMID:40993127<ref>PMID:40993127</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Brunger | <div class="pdbe-citations 9ojj" style="background-color:#fffaf0;"></div> | ||
[[Category: White | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rattus norvegicus]] | |||
[[Category: Brunger AT]] | |||
[[Category: White KI]] | |||
