9vcd: Difference between revisions

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New page: '''Unreleased structure''' The entry 9vcd is ON HOLD Authors: Dukhalin, S.D., Mariasina, S.S., Polshakov, V.I., Bocharov, E.V., Balagurov, K.I. Description: N-terminal domain of Drosop...
 
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'''Unreleased structure'''


The entry 9vcd is ON HOLD
==N-terminal dimeric C2H2-domain of Drosophila melanogaster IMZF protein==
<StructureSection load='9vcd' size='340' side='right'caption='[[9vcd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vcd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VCD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR,  models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vcd OCA], [https://pdbe.org/9vcd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vcd RCSB], [https://www.ebi.ac.uk/pdbsum/9vcd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vcd ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
C2H2 proteins, characterized by DNA-binding C2H2-type zinc finger domains, constitute the largest group of transcription factors. In addition to binding DNA, C2H2 domains can mediate protein-protein interactions, facilitating the oligomerization of C2H2 proteins. In this study, we identified eight C2H2 proteins in the Drosophila genome that feature a unique single C2H2 domain containing a conserved "CGxPhi" motif. Yeast two-hybrid assays, size-exclusion chromatography coupled with multi-angle light scattering, and chemical cross-linking experiments revealed a strong propensity of these domains to form dimers. Using NMR spectroscopy, we determined the solution structure of the dimeric C2H2 domain from the IMZF (Immune-mediated Zinc Finger) protein, providing structural evidence for the dimerization of C2H2 domains. Dimerization is mediated by the interface between the core C2H2 fold and the adjacent beta-strand containing the CGxPhi motif, which was further validated by structure-guided mutagenesis. A bioinformatic survey showed that "CGxPhi"-type C2H2 domains are specific to Diptera. Finally, our predictions demonstrate that dimerizing C2H2 domains containing additional structural elements could be widespread among eukaryotic taxa, with the highest prevalence in insects. These findings establish that single C2H2 domains can mediate self-association and identify the CGxPhi-type C2H2 domains as a distinct structural subclass specific to dipteran insects.


Authors: Dukhalin, S.D., Mariasina, S.S., Polshakov, V.I., Bocharov, E.V., Balagurov, K.I.
Beyond DNA binding: single C2H2 zinc fingers with adjacent beta-strands mediate dimerization in Drosophila transcription factors.,Balagurov KI, Mariasina SS, Dukhalin SD, Sluchanko NN, Golovnina AA, Khrustaleva AM, Maksimenko OG, Arkova OV, Stepanenko AA, Bocharov EV, Polshakov VI, Georgiev PG, Bonchuk AN Nucleic Acids Res. 2026 Jan 5;54(1):gkaf1425. doi: 10.1093/nar/gkaf1425. PMID:41495890<ref>PMID:41495890</ref>


Description: N-terminal domain of Drosophila melanogaster architectural protein CG18262
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Dukhalin, S.D]]
<div class="pdbe-citations 9vcd" style="background-color:#fffaf0;"></div>
[[Category: Bocharov, E.V]]
== References ==
[[Category: Mariasina, S.S]]
<references/>
[[Category: Balagurov, K.I]]
__TOC__
[[Category: Polshakov, V.I]]
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Balagurov KI]]
[[Category: Bocharov EV]]
[[Category: Dukhalin SD]]
[[Category: Mariasina SS]]
[[Category: Polshakov VI]]
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