9ryp: Difference between revisions
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New page: '''Unreleased structure''' The entry 9ryp is ON HOLD Authors: Koziej, L., Azuma, Y. Description: Aquifex aeolicus lumazine synthase R29A mutant 12-pentamer cage [[Category: Unreleased ... |
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The | ==Aquifex aeolicus lumazine synthase R29A mutant 12-pentamer cage== | ||
<StructureSection load='9ryp' size='340' side='right'caption='[[9ryp]], [[Resolution|resolution]] 1.71Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9ryp]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9RYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9RYP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 1.71Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ryp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ryp OCA], [https://pdbe.org/9ryp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ryp RCSB], [https://www.ebi.ac.uk/pdbsum/9ryp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ryp ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RISB_AQUAE RISB_AQUAE] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.<ref>PMID:12603336</ref> <ref>PMID:11237620</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Encapsulating metabolic enzymes within protein cages enhances catalytic efficiency through substrate channeling. The vitamin B2 biosynthesis system, in which a dodecahedral lumazine synthase (LS) cage encapsulates a homotrimeric riboflavin synthase (RS), exemplifies this strategy, yet the molecular basis for this stoichiometric enzyme encapsulation has remained elusive. Here, cryogenic electron microscopy structures reveal a hierarchical assembly mechanism that ensures the defined host-guest ratio. RS C-terminal cage-localization signal peptides anchor at LS pentamer-pentamer interfaces early during assembly, stabilizing open intermediates that, together with delayed later-stage cage closure, extend the loading window until guest incorporation is complete. RS spatial occupancy avoids overloading, while a molecular lock upon final closure prevents disassembly. The elucidated anchoring mechanism enabled structure-based phylogenetic analysis across diverse organisms, suggesting multiple independent evolutionary origins of this modular encapsulation strategy. This naturally occurring architecture provides design principles for engineering synthetic catalytic compartments with programmable stoichiometric control. | |||
A molecular basis for stoichiometric enzyme encapsulation in the vitamin B2 biosynthesis compartment.,Koziej L, Pankowski J, Stefanska M, Jankowski D, Gawin A, Malolan VV, Huiskonen JT, Kosugi T, Azuma Y Nat Commun. 2026 May 16. doi: 10.1038/s41467-026-73260-4. PMID:42143052<ref>PMID:42143052</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Azuma | <div class="pdbe-citations 9ryp" style="background-color:#fffaf0;"></div> | ||
[[Category: Koziej | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aquifex aeolicus VF5]] | |||
[[Category: Large Structures]] | |||
[[Category: Azuma Y]] | |||
[[Category: Koziej L]] | |||
Latest revision as of 05:00, 27 May 2026
Aquifex aeolicus lumazine synthase R29A mutant 12-pentamer cage
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