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New page: left|200px<br /> <applet load="1zl8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zl8" /> '''NMR structure of L27 heterodimer from C. el...
 
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[[Image:1zl8.gif|left|200px]]<br />
<applet load="1zl8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zl8" />
'''NMR structure of L27 heterodimer from C. elegans Lin-7 and H. sapiens Lin-2 scaffold proteins'''<br />


==Overview==
==NMR structure of L27 heterodimer from C. elegans Lin-7 and H. sapiens Lin-2 scaffold proteins==
LIN-2/7 (L27) domains are protein interaction modules that preferentially, hetero-oligomerize, a property critical for their function in directing, specific assembly of supramolecular signaling complexes at synapses and, other polarized cell-cell junctions. We have solved the solution structure, of the heterodimer composed of the L27 domains from LIN-2 and LIN-7., Comparison of this structure with other L27 domain structures has allowed, us to formulate a general model for why most L27 domains form an obligate, heterodimer complex. L27 domains can be divided in two types (A and B), with each heterodimer comprising an A/B pair. We have identified two, keystone positions that play a central role in discrimination. The, residues at these positions are energetically acceptable in the context of, an A/B heterodimer, but would lead to packing defects or electrostatic, repulsion in the context of A/A and B/B homodimers. As predicted by the, model, mutations of keystone residues stabilize normally strongly, disfavored homodimers. Thus, L27 domains are specifically optimized to, avoid homodimeric interactions.
<StructureSection load='1zl8' size='340' side='right'caption='[[1zl8]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZL8 FirstGlance]. <br>
1ZL8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZL8 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zl8 OCA], [https://pdbe.org/1zl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1zl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zl8 ProSAT]</span></td></tr>
==Reference==
</table>
A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes., Petrosky KY, Ou HD, Lohr F, Dotsch V, Lim WA, J Biol Chem. 2005 Nov 18;280(46):38528-36. Epub 2005 Sep 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16147993 16147993]
== Function ==
[https://www.uniprot.org/uniprot/P90976_CAEEL P90976_CAEEL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zl8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zl8 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Dotsch, V.]]
[[Category: Dotsch V]]
[[Category: Lim, W.A.]]
[[Category: Lim WA]]
[[Category: Lohr, F.]]
[[Category: Lohr F]]
[[Category: Ou, H.D.]]
[[Category: Ou HD]]
[[Category: Petrosky, K.Y.]]
[[Category: Petrosky KY]]
[[Category: alpha helix]]
[[Category: assembly]]
[[Category: heterodimer]]
[[Category: l27]]
[[Category: scaffold]]
[[Category: signaling]]
[[Category: specificity]]
 
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Retrieved from "https://proteopedia.org/1zl8"