1wac: Difference between revisions

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[[Image:1wac.gif|left|200px]]
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{{STRUCTURE_1wac|  PDB=1wac  |  SCENE=  }}
'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''


==Back-priming mode of Phi6 RNA-dependent RNA polymerase==
<StructureSection load='1wac' size='340' side='right'caption='[[1wac]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wac]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [https://pdbe.org/1wac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB], [https://www.ebi.ac.uk/pdbsum/1wac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wac ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RDRP_BPPH6 RDRP_BPPH6]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.


==Overview==
Back-priming mode of phi6 RNA-dependent RNA polymerase.,Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773<ref>PMID:15659773</ref>
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
</div>
<div class="pdbe-citations 1wac" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
[[Category: Pseudomonas phage phi6]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Bamford, D H.]]
__TOC__
[[Category: Grimes, J M.]]
</StructureSection>
[[Category: Laurila, M R.L.]]
[[Category: Large Structures]]
[[Category: Salgado, P S.]]
[[Category: Pseudomonas virus phi6]]
[[Category: Stuart, D I.]]
[[Category: Bamford DH]]
[[Category: Phi6 rna-dependent rna polymerase]]
[[Category: Grimes JM]]
[[Category: Transcription]]
[[Category: Laurila MRL]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:22:49 2008''
[[Category: Salgado PS]]
[[Category: Stuart DI]]
Retrieved from "https://proteopedia.org/1wac"