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[[Image:1wae.gif|left|200px]]
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{{STRUCTURE_1wae|  PDB=1wae  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE'''


==Crystal structure of H129V Mutant of Alcaligenes Xylosoxidans Nitrite Reductase==
<StructureSection load='1wae' size='340' side='right'caption='[[1wae]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wae]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wae OCA], [https://pdbe.org/1wae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wae RCSB], [https://www.ebi.ac.uk/pdbsum/1wae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wae ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O68601_ALCXX O68601_ALCXX]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wae_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wae ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone.


==Overview==
Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase.,Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622<ref>PMID:15554622</ref>
Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1WAE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAE OCA].
</div>
<div class="pdbe-citations 1wae" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15554622 15554622]
*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Achromobacter xylosoxidans]]
[[Category: Achromobacter xylosoxidans]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Antonyuk, S V.]]
[[Category: Antonyuk SV]]
[[Category: Eady, R R.]]
[[Category: Eady RR]]
[[Category: Ellis, M J.]]
[[Category: Ellis MJ]]
[[Category: Hasnain, S S.]]
[[Category: Hasnain SS]]
[[Category: Sawers, G.]]
[[Category: Sawers G]]
[[Category: Strange, R W.]]
[[Category: Strange RW]]
[[Category: Copper protein]]
[[Category: Nitrite reductase]]
[[Category: Reductase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:23:03 2008''
Retrieved from "https://proteopedia.org/1wae"