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[[Image:1waj.gif|left|200px]]
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{{STRUCTURE_1waj|  PDB=1waj  |  SCENE=  }}
'''DNA POLYMERASE FROM BACTERIOPHAGE RB69'''


==DNA POLYMERASE FROM BACTERIOPHAGE RB69==
<StructureSection load='1waj' size='340' side='right'caption='[[1waj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1waj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_RB69 Escherichia phage RB69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1waj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1waj OCA], [https://pdbe.org/1waj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1waj RCSB], [https://www.ebi.ac.uk/pdbsum/1waj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1waj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DPOL_BPR69 DPOL_BPR69] This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1waj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1waj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.


==Overview==
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69.,Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631<ref>PMID:9215631</ref>
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1WAJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb69 Enterobacteria phage rb69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAJ OCA].
</div>
<div class="pdbe-citations 1waj" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9215631 9215631]
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
[[Category: DNA-directed DNA polymerase]]
== References ==
[[Category: Enterobacteria phage rb69]]
<references/>
[[Category: Single protein]]
__TOC__
[[Category: Karam, J D.]]
</StructureSection>
[[Category: Konigsberg, W H.]]
[[Category: Escherichia phage RB69]]
[[Category: Satter, A K.M A.]]
[[Category: Large Structures]]
[[Category: Steitz, T A.]]
[[Category: Karam JD]]
[[Category: Wang, C C.]]
[[Category: Konigsberg WH]]
[[Category: Wang, J.]]
[[Category: Satter AKMA]]
[[Category: Nucleotidyltransferase]]
[[Category: Steitz TA]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:23:10 2008''
[[Category: Wang CC]]
[[Category: Wang J]]
Retrieved from "https://proteopedia.org/1waj"