9vyc: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "9vyc" [edit=sysop:move=sysop] |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==The crystal structure of PaiB from Bacillus stearothermophilus bound to HEM== | ||
<StructureSection load='9vyc' size='340' side='right'caption='[[9vyc]], [[Resolution|resolution]] 2.42Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9vyc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VYC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4212272Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vyc OCA], [https://pdbe.org/9vyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vyc RCSB], [https://www.ebi.ac.uk/pdbsum/9vyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vyc ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nitrogen-nitrogen (N-N) bond formation is integrated into the biosynthetic pathways of various classes of natural products, some of which exhibit intriguing biological activities. While recent studies have identified several distinct groups of enzymes responsible for N-N bond formation, the underlying catalytic mechanisms are largely unknown. Here, we report the dimeric structure of the N-N bond forming enzyme LnzB (Streptomyces spp.), which relies on a heme-iron to catalyze the formation of intramolecular N-N bonds using N-hydroxyornithine as a substrate. The structure reveals the molecular architecture of its active sites and heme-interacting pocket. In combination with MD simulation, site-directed mutagenesis, and kinetic activity assays, we have identified key residues responsible for ligand binding and N-N bond formation activity. Phylogenetic analysis and structural comparison reveal that LnzB and its homologues may have evolved from the transcriptional regulator PaiB by altering the substrate binding pocket. Our study extends the limited knowledge of N-N bond formation catalyzed by a heme iron-dependent enzyme in natural products. | |||
Structural insights into heme-iron dependent N-N bond formation enzyme LnzB.,Huang H, Wang L, Chen P, Yang T, Zhu C, Li S, Zhou Y, Tan Y, Li Z, Zhang H, Chen J, Zhang ZM Commun Chem. 2025 Nov 10;8(1):344. doi: 10.1038/s42004-025-01724-7. PMID:41214184<ref>PMID:41214184</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Huang | <div class="pdbe-citations 9vyc" style="background-color:#fffaf0;"></div> | ||
[[Category: Zhang | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Geobacillus kaustophilus HTA426]] | |||
[[Category: Large Structures]] | |||
[[Category: Huang HS]] | |||
[[Category: Zhang ZM]] | |||
Latest revision as of 16:03, 1 April 2026
The crystal structure of PaiB from Bacillus stearothermophilus bound to HEM
| ||||||||||||
