9scf: Difference between revisions
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==Fe(II)-2-oxoglutarate-dependent Pseudomonas savastanoi pv phaseolicola 1449B in complex with 2-oxoglutarate== | |||
<StructureSection load='9scf' size='340' side='right'caption='[[9scf]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9scf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_savastanoi Pseudomonas savastanoi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SCF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9scf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9scf OCA], [https://pdbe.org/9scf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9scf RCSB], [https://www.ebi.ac.uk/pdbsum/9scf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9scf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pseudomonas savastanoi pv. phaseolicola PK2 employs an Fe(II)-dependent ethylene/succinate-forming enzyme (PK2 PsEFE) to produce ethylene from 2-oxoglutarate (2OG). Here we report NMR-based assays showing that the putative P. savastanoi pv. glycinea PsEFE, which differs from PK2 PsEFE by a single residue, and the P. savastanoi pv.1449B PsEFE, which differs from PK2 PsEFE by 28 residues and a C-terminal 13-residue truncation, catalyze ethylene production from 2OG. Like the PK2 PsEFE, they catalyze oxidation of naturally occurring 2OG derivatives to give alcohol and diacid products. Crystallographic analysis demonstrates that the overall fold and active site of 1449B PsEFE is similar to that of PK2 PsEFE. Interestingly, 2OG was observed to adopt an atypical inverse metal ion binding mode in complex with 1449B PsEFE:Mn in which its 2-oxoacid group is positioned to interact with the guanidinium group of R277, but not the Mn ion, which substitutes for catalytically active Fe(II). Together with reported crystallographic results, this observation indicates that 2OG metal ion binding modes and conformations at the active sites of 2OG oxygenases can vary, possibly in a functionally or disease relevant manner. | |||
Structural and Functional Validation of Pseudomonas Savastanoi Ethylene Forming Enzymes Reveals Flexibility in 2-Oxoglutarate Binding Mode and Conformation.,Sun Y, Dhingra S, Allen MD, Cheng ESZ, Zhang Z, Schofield CJ, Brewitz L Chembiochem. 2026 Mar 13;27(5):e202500702. doi: 10.1002/cbic.202500702. PMID:41797450<ref>PMID:41797450</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9scf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas savastanoi]] | |||
[[Category: Allen M]] | |||
[[Category: Brewitz L]] | |||
[[Category: Dhingra S]] | |||
[[Category: Schofield CJ]] | |||
[[Category: Sun Y]] | |||
Latest revision as of 07:43, 19 March 2026
Fe(II)-2-oxoglutarate-dependent Pseudomonas savastanoi pv phaseolicola 1449B in complex with 2-oxoglutarate
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