9q75: Difference between revisions
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New page: '''Unreleased structure''' The entry 9q75 is ON HOLD Authors: Penteado, R.F., Guimaraes, B.G. Description: Crystal structure of T. cruzi EIF4E5 in complex with EIF4G1 peptide and cap-4... |
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==Crystal structure of T. cruzi EIF4E5 in complex with EIF4G1 peptide and cap-4.== | |||
<StructureSection load='9q75' size='340' side='right'caption='[[9q75]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9q75]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_Dm28c Trypanosoma cruzi Dm28c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Q75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Q75 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LRM:[(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-2-[[[(2~{R},3~{R},4~{R},5~{R})-2-[[[[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-5-[6-(dimethylamino)purin-9-yl]-4-methoxy-oxolan-3-yl]oxy-oxidanyl-phosphoryl]oxymethyl]-4-methoxy-oxolan-3-yl]+[(2~{R},3~{R},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-4-methoxy-3-[[(2~{R},3~{R},4~{R},5~{R})-4-methoxy-5-[3-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxolan-2-yl]methyl+hydrogen+phosphate'>LRM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9q75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9q75 OCA], [https://pdbe.org/9q75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9q75 RCSB], [https://www.ebi.ac.uk/pdbsum/9q75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9q75 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A2V2XI07_TRYCR A0A2V2XI07_TRYCR] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Translation initiation in eukaryotes is a highly regulated process involving the assembly of several transient protein complexes. A key step in this process is recognition of the mRNA 5' cap structure by the initiation factor eIF4E, a core component of the eIF4F complex. In trypanosomatids, this mechanism diverges from canonical eukaryotic systems, featuring five distinct eIF4F-like complexes formed through specific pairings of eIF4E and eIF4G homologs. Additionally, trypanosomatid mRNAs exhibit a unique hypermethylated cap-4 structure at the 5' end. To elucidate the molecular basis of selective eIF4E-eIF4G interactions and the modulation of cap binding upon eIF4G engagement, we determined high-resolution crystal structures of EIF4E5-EIF4G1 complexes from Trypanosoma brucei and T. cruzi, and the EIF4E6-EIF4G5 complex from T. cruzi. These structural studies, supported by biophysical analyses in the presence and absence of a cap-4 analog, reveal key determinants of cap recognition associated with cap-4 structural flexibility and plasticity in the cap-binding pockets. We observe conformational rearrangements upon eIF4G binding and propose a relationship between these structural changes and increased cap-4 affinity. In addition, comparative structural analysis of the EIF4E5-EIF4G1 and EIF4E6-EIF4G5 complexes offers atomic-level insights into the molecular determinants of specificity that govern selective eIF4E-eIF4G pairings in trypanosomatids. | |||
Selective eIF4E-eIF4G Pairing and Cap-4 Recognition Mechanisms in Trypanosomatids: Insights From EIF4E5-EIF4G1 and EIF4E6-EIF4G5 Complexes.,Penteado RF, Vichier-Guerre S, da Silva Pereira BM, Dugue L, Guerra Slompo EP, Assuncao de Matos TR, Pochet S, Zanchin NIT, Guimaraes BG J Mol Biol. 2026 Jan 15;438(2):169550. doi: 10.1016/j.jmb.2025.169550. Epub 2025 , Nov 18. PMID:41265746<ref>PMID:41265746</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9q75" style="background-color:#fffaf0;"></div> | ||
[[Category: Guimaraes | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Trypanosoma cruzi Dm28c]] | |||
[[Category: Guimaraes BG]] | |||
[[Category: Penteado RF]] | |||
