9wrj: Difference between revisions

Latest revision as of 14:47, 10 February 2026

Crystal structure of CtBP1 in complex with PALI1

Structural highlights

9wrj is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	NAD
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CTBP1_HUMAN Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Polycomb Repressive Complex 2 (PRC2) and C-terminal binding proteins 1 and 2 (CtBP1/2) are key epigenetic regulators that frequently co-occupy chromatin, yet their crosstalk remains poorly understood. Here, we delineate the molecular basis of the interaction between CtBP1/2 and the N-terminal domain of PALI1, an accessory subunit of PRC2. The PALI1 N-terminus contains two DLS-like motifs that bind CtBP1/2 bivalently, enhancing affinity and promoting higher-order oligomerization. Conversely, disruption of CtBP1/2 oligomerization weakens PALI1 binding, revealing a reciprocal mechanism stabilizing the CtBP1/2-PALI1 complex. The 2.20 A structure of the CtBP1-PALI1 complex reveals the detailed interaction interface, which, together with biochemical data, supports a model where tandem DLS-like motifs drive CtBP1/2 oligomerization and multivalent engagement. Through its C-terminal PRC2-binding domain, PALI1 acts as a dual-interface scaffold linking CtBP1/2 and PRC2, providing a structural framework for their coordinated chromatin recruitment and transcriptional repression.

PALI1 enhances CtBP1/2 oligomerization and couples CtBP1/2 to PRC2.,Zhang B, Jiang J, Chen P, Lin C, Sun W, Wang J, Li W, Chen J, Luo Q, Cai D, Cai Q, Chen S Biochem Biophys Res Commun. 2026 Feb 12;800:153295. doi: , 10.1016/j.bbrc.2026.153295. Epub 2026 Jan 14. PMID:41547303^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sewalt RG, Gunster MJ, van der Vlag J, Satijn DP, Otte AP. C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins. Mol Cell Biol. 1999 Jan;19(1):777-87. PMID:9858600
↑ Alpatov R, Munguba GC, Caton P, Joo JH, Shi Y, Shi Y, Hunt ME, Sugrue SP. Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene. Mol Cell Biol. 2004 Dec;24(23):10223-35. PMID:15542832 doi:https://dx.doi.org/10.1128/MCB.24.23.10223-10235.2004
↑ Purbey PK, Singh S, Notani D, Kumar PP, Limaye AS, Galande S. Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1. Mol Cell Biol. 2009 Mar;29(5):1321-37. doi: 10.1128/MCB.00822-08. Epub 2008 Dec, 22. PMID:19103759 doi:10.1128/MCB.00822-08
↑ Kumar V, Carlson JE, Ohgi KA, Edwards TA, Rose DW, Escalante CR, Rosenfeld MG, Aggarwal AK. Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase. Mol Cell. 2002 Oct;10(4):857-69. PMID:12419229
↑ Zhang B, Jiang J, Chen P, Lin C, Sun W, Wang J, Li W, Chen J, Luo Q, Cai D, Cai Q, Chen S. PALI1 enhances CtBP1/2 oligomerization and couples CtBP1/2 to PRC2. Biochem Biophys Res Commun. 2026 Feb 12;800:153295. PMID:41547303 doi:10.1016/j.bbrc.2026.153295

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OCA

[1] Sewalt RG, Gunster MJ, van der Vlag J, Satijn DP, Otte AP. C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins. Mol Cell Biol. 1999 Jan;19(1):777-87. PMID:9858600

[2] Alpatov R, Munguba GC, Caton P, Joo JH, Shi Y, Shi Y, Hunt ME, Sugrue SP. Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene. Mol Cell Biol. 2004 Dec;24(23):10223-35. PMID:15542832 doi:https://dx.doi.org/10.1128/MCB.24.23.10223-10235.2004

[3] Purbey PK, Singh S, Notani D, Kumar PP, Limaye AS, Galande S. Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1. Mol Cell Biol. 2009 Mar;29(5):1321-37. doi: 10.1128/MCB.00822-08. Epub 2008 Dec, 22. PMID:19103759 doi:10.1128/MCB.00822-08

[4] Kumar V, Carlson JE, Ohgi KA, Edwards TA, Rose DW, Escalante CR, Rosenfeld MG, Aggarwal AK. Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase. Mol Cell. 2002 Oct;10(4):857-69. PMID:12419229

[5] Zhang B, Jiang J, Chen P, Lin C, Sun W, Wang J, Li W, Chen J, Luo Q, Cai D, Cai Q, Chen S. PALI1 enhances CtBP1/2 oligomerization and couples CtBP1/2 to PRC2. Biochem Biophys Res Commun. 2026 Feb 12;800:153295. PMID:41547303 doi:10.1016/j.bbrc.2026.153295

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[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9wrj is ON HOLD
+==Crystal structure of CtBP1 in complex with PALI1==
+<StructureSection load='9wrj' size='340' side='right'caption='[[9wrj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9wrj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WRJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WRJ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wrj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wrj OCA], [https://pdbe.org/9wrj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wrj RCSB], [https://www.ebi.ac.uk/pdbsum/9wrj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wrj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CTBP1_HUMAN CTBP1_HUMAN] Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.<ref>PMID:9858600</ref> <ref>PMID:15542832</ref> <ref>PMID:19103759</ref> <ref>PMID:12419229</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Polycomb Repressive Complex 2 (PRC2) and C-terminal binding proteins 1 and 2 (CtBP1/2) are key epigenetic regulators that frequently co-occupy chromatin, yet their crosstalk remains poorly understood. Here, we delineate the molecular basis of the interaction between CtBP1/2 and the N-terminal domain of PALI1, an accessory subunit of PRC2. The PALI1 N-terminus contains two DLS-like motifs that bind CtBP1/2 bivalently, enhancing affinity and promoting higher-order oligomerization. Conversely, disruption of CtBP1/2 oligomerization weakens PALI1 binding, revealing a reciprocal mechanism stabilizing the CtBP1/2-PALI1 complex. The 2.20 A structure of the CtBP1-PALI1 complex reveals the detailed interaction interface, which, together with biochemical data, supports a model where tandem DLS-like motifs drive CtBP1/2 oligomerization and multivalent engagement. Through its C-terminal PRC2-binding domain, PALI1 acts as a dual-interface scaffold linking CtBP1/2 and PRC2, providing a structural framework for their coordinated chromatin recruitment and transcriptional repression.
-Authors:
+PALI1 enhances CtBP1/2 oligomerization and couples CtBP1/2 to PRC2.,Zhang B, Jiang J, Chen P, Lin C, Sun W, Wang J, Li W, Chen J, Luo Q, Cai D, Cai Q, Chen S Biochem Biophys Res Commun. 2026 Feb 12;800:153295. doi: , 10.1016/j.bbrc.2026.153295. Epub 2026 Jan 14. PMID:41547303<ref>PMID:41547303</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 9wrj" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Cai Q]]
+[[Category: Chen S]]
+[[Category: Jiang J]]
+[[Category: Sun W]]
+[[Category: Zhang B]]

9wrj: Difference between revisions

Latest revision as of 14:47, 10 February 2026

Crystal structure of CtBP1 in complex with PALI1

Structural highlights

Function

Publication Abstract from PubMed

References

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