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| <!--
| | ==Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (1.50 angstrom, 293 K)== |
| The line below this paragraph, containing "STRUCTURE_1wme", creates the "Structure Box" on the page.
| | <StructureSection load='1wme' size='340' side='right'caption='[[1wme]], [[Resolution|resolution]] 1.50Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1wme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._KSM-KP43 Bacillus sp. KSM-KP43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WME FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| -->
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| {{STRUCTURE_1wme| PDB=1wme | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wme OCA], [https://pdbe.org/1wme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wme RCSB], [https://www.ebi.ac.uk/pdbsum/1wme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wme ProSAT]</span></td></tr> |
| | | </table> |
| '''Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (1.50 angstrom, 293 K)'''
| | == Function == |
| | | [https://www.uniprot.org/uniprot/Q93UV9_9BACI Q93UV9_9BACI] |
| | | == Evolutionary Conservation == |
| ==Overview== | | [[Image:Consurf_key_small.gif|200px|right]] |
| The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43 from Bacillus sp. KSM-KP43, with a C-terminal extension domain, was determined by the multiple isomorphous replacements method with anomalous scattering. The native form was refined to a crystallographic R factor of 0.134 (Rfree of 0.169) at 1.30-A resolution. KP-43 consists of two domains, a subtilisin-like alpha/beta domain and a C-terminal jelly roll beta-barrel domain. The topological architecture of the molecule is similar to that of kexin and furin, which belong to the subtilisin-like proprotein convertases, whereas the amino acid sequence and the binding orientation of the C-terminal beta-barrel domain both differ in each case. Since the C-terminal domains of subtilisin-like proprotein convertases are essential for folding themselves, the domain of KP-43 is also thought to play such a role. KP-43 is known to be an oxidation-resistant protease among the general subtilisin-like proteases. To investigate how KP-43 resists oxidizing reagents, the structure of oxidized KP-43 was also determined and refined to a crystallographic R factor of 0.142 (Rfree of 0.212) at 1.73-A resolution. The structure analysis revealed that Met-256, adjacent to catalytic Ser-255, was oxidized similarly to an equivalent residue in subtilisin BPN'. Although KP-43, as well as proteinase K and subtilisin Carlsberg, lose their hydrolyzing activity against synthetic peptides after oxidation treatment, all of them retain 70-80% activity against proteinaceous substrates. These results, as well as the beta-casein digestion pattern analysis, have indicated that the oxidation of the methionine adjacent to the catalytic serine is not a dominant modification but might alter the substrate specificities.
| | Check<jmol> |
| | | <jmolCheckbox> |
| ==About this Structure== | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wm/1wme_consurf.spt"</scriptWhenChecked> |
| 1WME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WME OCA].
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | | <text>to colour the structure by Evolutionary Conservation</text> |
| ==Reference== | | </jmolCheckbox> |
| The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain., Nonaka T, Fujihashi M, Kita A, Saeki K, Ito S, Horikoshi K, Miki K, J Biol Chem. 2004 Nov 5;279(45):47344-51. Epub 2004 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15342641 15342641]
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wme ConSurf]. |
| [[Category: Bacteria]] | | <div style="clear:both"></div> |
| [[Category: Single protein]] | | __TOC__ |
| [[Category: Fujihashi, M.]] | | </StructureSection> |
| [[Category: Horikoshi, K.]] | | [[Category: Bacillus sp. KSM-KP43]] |
| [[Category: Ito, S.]] | | [[Category: Large Structures]] |
| [[Category: Kita, A.]] | | [[Category: Fujihashi M]] |
| [[Category: Miki, K.]] | | [[Category: Horikoshi K]] |
| [[Category: Nonaka, T.]] | | [[Category: Ito S]] |
| [[Category: Saeki, K.]] | | [[Category: Kita A]] |
| [[Category: Alpha-beta hydrolase fold]]
| | [[Category: Miki K]] |
| [[Category: Jelly-roll beta-barrel]]
| | [[Category: Nonaka T]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:52:23 2008''
| | [[Category: Saeki K]] |
