9wsf: Difference between revisions
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New page: '''Unreleased structure''' The entry 9wsf is ON HOLD Authors: Nln, S., Kumar, V., Sahoo, P.K., Jain, D. Description: Crystal structure of Sigma28/FlgM complex from Pseudomonas aerugino... |
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The | ==Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution== | ||
<StructureSection load='9wsf' size='340' side='right'caption='[[9wsf]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9wsf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WSF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wsf OCA], [https://pdbe.org/9wsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wsf RCSB], [https://www.ebi.ac.uk/pdbsum/9wsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wsf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Late flagellar genes in Pseudomonas aeruginosa are transcribed by the group 3 sigma factor, FliA (sigma28). sigma28 drives the expression of flagellin, which assembles into the flagellar filament in this monoflagellated bacterium. This function is suppressed by the anti-sigma factor FlgM. Here, we present the 1.95A resolution crystal structure of sigma28-FlgM complex, along with a 3.4A structure of sigma28RNAP open promoter complex determined using single particle cryo-electron microscopy from P. aeruginosa. The sigma28 adopts a compact conformation upon binding to the anti-sigma factor FlgM, which contacts all three domains of the sigma factor. This conformation is neither conducive to interactions with RNA polymerase nor the promoter DNA. The cryo-EM structure reveals base-specific interactions of sigma28 domain 4 (sigma4) with -35 element, flipping of -11 base of the template strand, novel interactions of template strand with domain 2 (sigma2) and 3 (sigma3), and partial insertion of sigma finger into the active site cleft, offering unique features of group 3 sigma interactions with promoter DNA. Perturbation of key residues affects transcription in vitro and flagellar phenotypes as well as bacterial motility in vivo. Analysis of the structural data presented here reveals new insights into transcription regulation of late flagellar genes. | |||
Structural insights into sigma28-dependent transcription initiation and its regulation by anti-sigma factor in Pseudomonas aeruginosa.,Sheenu, Kumar V, Sahoo PK, Kandiah E, Jain D Nucleic Acids Res. 2026 Jan 5;54(1):gkaf1414. doi: 10.1093/nar/gkaf1414. PMID:41521663<ref>PMID:41521663</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9wsf" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa]] | |||
[[Category: Jain D]] | |||
[[Category: Kumar V]] | |||
[[Category: Nln S]] | |||
[[Category: Sahoo PK]] | |||
Latest revision as of 07:43, 25 February 2026
Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution
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