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New page: left|200px<br /> <applet load="1zzi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zzi, resolution 1.80Å" /> '''Crystal Structure A...
 
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[[Image:1zzi.gif|left|200px]]<br />
<applet load="1zzi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zzi, resolution 1.80&Aring;" />
'''Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA'''<br />


==Overview==
==Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA==
The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in, multiple functions in the regulation of gene expression and acts as a hub, at the intersection of signaling pathways and processes involving nucleic, acids. Central to its function is its ability to bind both ssDNA and ssRNA, via its KH (hnRNP K homology) domains. We determined crystal structures of, hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4, and 1.8 A resolution, respectively, and show that the KH3 domain binds, specifically to both TCCC and CCCC sequences. In parallel, we used NMR to, compare the binding affinity and mode of interaction of the KH3 domain, with several ssRNA ligands and CTC(4) ssDNA. Based on a structure, alignment of the KH3-CTC(4) complex with known structures of other KH, domains in complex with ssRNA, we discuss recognition of tetranucleotide, sequences by KH domains.
<StructureSection load='1zzi' size='340' side='right'caption='[[1zzi]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zzi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZZI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzi OCA], [https://pdbe.org/1zzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zzi RCSB], [https://www.ebi.ac.uk/pdbsum/1zzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zzi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HNRPK_HUMAN HNRPK_HUMAN] One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.<ref>PMID:16360036</ref> <ref>PMID:20673990</ref> <ref>PMID:22825850</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/1zzi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zzi ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1ZZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA].
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
 
== References ==
==Reference==
<references/>
X-ray crystallographic and NMR studies of the third KH domain of hnRNP K in complex with single-stranded nucleic acids., Backe PH, Messias AC, Ravelli RB, Sattler M, Cusack S, Structure. 2005 Jul;13(7):1055-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16004877 16004877]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Backe, P.H.]]
[[Category: Backe PH]]
[[Category: Cusack, S.]]
[[Category: Cusack S]]
[[Category: Messias, A.C.]]
[[Category: Messias AC]]
[[Category: Ravelli, R.B.]]
[[Category: Ravelli RB]]
[[Category: Sattler, M.]]
[[Category: Sattler M]]
[[Category: protein-ssdna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:43:22 2007''

Latest revision as of 13:39, 13 March 2024

Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA

1zzi, resolution 1.80Å

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