9x91: Difference between revisions
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New page: '''Unreleased structure''' The entry 9x91 is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Solution structure of anti-CRISPR AcrIIA8== | |||
<StructureSection load='9x91' size='340' side='right'caption='[[9x91]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9x91]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X91 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x91 OCA], [https://pdbe.org/9x91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x91 RCSB], [https://www.ebi.ac.uk/pdbsum/9x91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x91 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A447EB47_9ZZZZ A0A447EB47_9ZZZZ] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Anti-CRISPR (Acr) proteins are phage-encoded anti-defense factors that suppress CRISPR-Cas immunity in bacteria. AcrIIA8 was previously identified as an inhibitor of Streptococcus pyogenes Cas9 (SpyCas9) through functional assays of metagenomic libraries. Here, we report that AcrIIA8 does not inhibit SpyCas9 in biochemical assays under a range of buffer conditions and temperatures. The solution structure and dynamics of AcrIIA8 reveal a six-stranded beta-barrel fold with flexible beta1-beta2 and beta2-beta3 loops, characteristic of phage virion-assembly proteins. In addition, genomic context analysis places AcrIIA8 and its homologs within conserved prophage morphogenetic regions at the position expected for type II head-tail joining (HTJ2) proteins. We further detected no interaction between AcrIIA8 and SpyCas9 in NMR titration experiments, suggesting that they do not specifically associate. Taken together, these findings argue against assigning AcrIIA8 as a SpyCas9 inhibitor and instead support its annotation as a putative phage structural protein of the HTJ2 family. | |||
AcrIIA8 is a putative phage structural protein of the HTJ2 family that does not inhibit Streptococcus pyogenes Cas9.,An SY, Kim I, Hong SH, Kim EH, Suh JY Protein Sci. 2026 Jul;35(7):e70651. doi: 10.1002/pro.70651. PMID:42223080<ref>PMID:42223080</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9x91" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Metagenome]] | |||
[[Category: An SY]] | |||
[[Category: Hong SH]] | |||
[[Category: Kim I]] | |||
[[Category: Suh JY]] | |||
Latest revision as of 04:59, 24 June 2026
Solution structure of anti-CRISPR AcrIIA8
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