9x86: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 9x86 is ~~ON HOLD until Paper~~ Publication

==Crystal Structure of dehydratase ApmL in Amipurimycin biosynthesis==

== Structural highlights ==

<table><tr><td colspan='2'>[[9x86]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces Streptomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X86 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x86 OCA], [https://pdbe.org/9x86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x86 RCSB], [https://www.ebi.ac.uk/pdbsum/9x86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x86 ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Amipurimycin is a peptidyl nucleoside antibiotic characterized by a C(9) high-carbon sugar, with potent activity against the rice blast pathogen Pyricularia oryzae. The biosynthetic machinery of the C-4' methylene group in its deoxy sugar has remained unknown. This study reveals that ApmL, a hypothetical protein in the DUF3500 family, functions as a 4',5'-dehydratase whose activity is strictly dependent on the PKS assembly line. Together with its partner reductase ApmM, they complete a two-step C-4' deoxygenation process via dehydration-reduction. We achieve the first in vitro reconstitution of this unique PKS-coupled dehydration process and show direct interaction between ApmL and PKS proteins. Our findings establish ApmL as a new family of dehydratase and uncover a noncanonical deoxygenation strategy in nucleoside antibiotic biosynthesis.

~~Authors~~:

Deoxysugar Formation via 4',5'-Dehydration on PKS Assembly Line in Nucleoside Antibiotic Biosynthesis.,Chen ZH, Wang F, Wang W, Zhang TC, Wang YL, Zhang WH, Pu J, Sun A, Pan HX, Tang GL Angew Chem Int Ed Engl. 2026 Mar 28:e6927983. doi: 10.1002/anie.6927983. PMID:41902592<ref>PMID:41902592</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Streptomyces]]

[[Category: Sun A]]

[[Category: Wang W]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9x86 is ON HOLD  until Paper Publication
+==Crystal Structure of dehydratase ApmL in Amipurimycin biosynthesis==
+<StructureSection load='9x86' size='340' side='right'caption='[[9x86]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9x86]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces Streptomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X86 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x86 OCA], [https://pdbe.org/9x86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x86 RCSB], [https://www.ebi.ac.uk/pdbsum/9x86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x86 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Amipurimycin is a peptidyl nucleoside antibiotic characterized by a C(9) high-carbon sugar, with potent activity against the rice blast pathogen Pyricularia oryzae. The biosynthetic machinery of the C-4' methylene group in its deoxy sugar has remained unknown. This study reveals that ApmL, a hypothetical protein in the DUF3500 family, functions as a 4',5'-dehydratase whose activity is strictly dependent on the PKS assembly line. Together with its partner reductase ApmM, they complete a two-step C-4' deoxygenation process via dehydration-reduction. We achieve the first in vitro reconstitution of this unique PKS-coupled dehydration process and show direct interaction between ApmL and PKS proteins. Our findings establish ApmL as a new family of dehydratase and uncover a noncanonical deoxygenation strategy in nucleoside antibiotic biosynthesis.
-Authors:
+Deoxysugar Formation via 4',5'-Dehydration on PKS Assembly Line in Nucleoside Antibiotic Biosynthesis.,Chen ZH, Wang F, Wang W, Zhang TC, Wang YL, Zhang WH, Pu J, Sun A, Pan HX, Tang GL Angew Chem Int Ed Engl. 2026 Mar 28:e6927983. doi: 10.1002/anie.6927983. PMID:41902592<ref>PMID:41902592</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 9x86" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces]]
+[[Category: Sun A]]
+[[Category: Wang W]]