9w1l: Difference between revisions
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==NMR Structure of the 5BSL3.2 Apical Stem-Loop RNA from HCV== | |||
<StructureSection load='9w1l' size='340' side='right'caption='[[9w1l]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9w1l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_(isolate_1) Hepatitis C virus (isolate 1)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9W1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9W1L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9w1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9w1l OCA], [https://pdbe.org/9w1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9w1l RCSB], [https://www.ebi.ac.uk/pdbsum/9w1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9w1l ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hepatitis C virus (HCV) is a major global health burden, associated with chronic liver diseases, including cirrhosis and hepatocellular carcinoma. Viral replication critically depends on conserved cis-acting replication elements (CREs), such as the 5BSL3.2 stem-loop near the 3' end of the open reading frame. This element forms a long-range kissing-loop interaction with the SL2 domain of the 3'X tail, essential for efficient genome replication. However, the role of host RNA-binding proteins (RBPs) in regulating this RNA-RNA interaction remains poorly understood. To explore this, we investigated whether the host RBP hnRNPA1 modulates HCV replication by targeting the 5BSL3.2 element. Using an integrated approach combining structural biology, biophysics, and biochemical assays, we identify the terminal loop of 5BSL3.2 as a high-affinity binding site for the tandem RNA recognition motifs (RRMs) of hnRNPA1. Our data reveal that adenine-rich residues within the loop are critical for binding specificity. Our results uncover a structural mechanism by which hnRNPA1 binding perturbs the kissing-loop interaction between 5BSL3.2 and the SL2 element of the viral 3'X-tail, which impacts viral replication. This study highlights a previously unrecognized role of hnRNPA1 in modulating viral RNA structure and suggests a novel interface for host-directed antiviral intervention. | |||
Loop of fate: structural and mechanistic insights into hnRNPA1 binding to the hepatitis C virus RNA.,Kumar A, Penumutchu S, Panchariya L, Kumari P, Thakur S, Daripa P, Singh V, Arulandu A, Maiti S, Deshmukh MV, Jain N RNA. 2026 Jan 16;32(2):215-236. doi: 10.1261/rna.080770.125. PMID:41285614<ref>PMID:41285614</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Deshmukh | <div class="pdbe-citations 9w1l" style="background-color:#fffaf0;"></div> | ||
[[Category: Jain | == References == | ||
[[Category: Kumar | <references/> | ||
[[Category: Penumutchu | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Deshmukh MV]] | |||
[[Category: Jain N]] | |||
[[Category: Kumar A]] | |||
[[Category: Penumutchu S]] | |||
Latest revision as of 14:47, 10 February 2026
NMR Structure of the 5BSL3.2 Apical Stem-Loop RNA from HCV
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