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| == A "Photoswitchable" HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
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| ==== Claire Deo, Franziska Walterspiel, Begoña Ugarte-Uribe ====
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| ==== [https://onlinelibrary.wiley.com/share/ZFYT2R9ZRCUTJRHQZCRW?target=10.1002/anie.202424955]. ([https://doi.org/10.1002/anie.202424955 DOI: 10.1002/anie.202424955])====
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| The Photoswitchable Halo Tag (psHaloTag) is a unique chemigenetic system designed to provide reversible, light-controlled fluorescence tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging in biological microscopy <ref>https://doi.org/10.1002/anie.202424955</ref>. psHaloTag addresses a major gap in the availability of reliable, reversible systems suitable for live-cell imaging. Many current systems are limited in tracking long-term, cyclical processes or in refreshing the pool of observable molecules. psHaloTag combines the genetically encoded HaloTag protein with the light-sensing sAsLOV2 domain. When illuminated with 450 nm light, psHaloTag undergoes a reversible change that activates a bound rhodamine dye ligand, resulting in a significant increase in fluorescence. This strong, multiple-cycle reporter overcomes the limitations of irreversible systems. It is an important tool for achieving precise spatiotemporal control in demanding applications such as live-cell Super-Resolution Microscopy (SMLM), where many established photosensitive probes cannot be reactivated.
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| ==== Function ====
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| ==== Mechanism ====
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| ==== Structural highlights ====
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| ==== Relevance ====
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| This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
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| </StructureSection>
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| == References ==
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| <references/>
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