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| ==APhotoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells==
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| <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
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| == Claire Deo, Franziska Walterspiel, Begoña Ugarte-Uribe ==
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| ==[https://onlinelibrary.wiley.com/share/ZFYT2R9ZRCUTJRHQZCRW?target=10.1002/anie.202424955]. ([https://doi.org/10.1002/anie.202424955 DOI: 10.1002/anie.202424955])==
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| The Photoswitchable Halo Tag (psHaloTag) is a unique chemigenetic system designed to provide reversible, light-controlled fluorescence tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging in biological microscopy <ref>https://doi.org/10.1002/anie.202424955</ref>. psHaloTag addresses a major gap in the availability of reliable, reversible systems suitable for live-cell imaging. Many current systems are limited in tracking long-term, cyclical processes or in refreshing the pool of observable molecules. psHaloTag combines the genetically encoded HaloTag protein with the light-sensing sAsLOV2 domain. When illuminated with 450 nm light, psHaloTag undergoes a reversible change that activates a bound rhodamine dye ligand, resulting in a significant increase in fluorescence. This strong, multiple-cycle reporter overcomes the limitations of irreversible systems. It is an important tool for achieving precise spatiotemporal control in demanding applications such as live-cell Super-Resolution Microscopy (SMLM), where many established photosensitive probes cannot be reactivated.
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| ==== Function ====
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| ----
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| The psHaloTag works as an allosteric photoswitch.
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| *Protein Scaffold: An engineered HaloTag protein with the light-sensing sAsLOV2 domain.
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| *Ligand: Binds to a fluorogenic rhodamine dye (e.g., JF635-HTL).
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| :Light ON (450 nm): Light activates the sAsLOV2 domain using FMN cofactor, causing a conformational change (Jα helix unfolding).
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| :Fluorescence ON: This change forces the bound dye into its fluorescent state.
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| :Light OFF (Dark): The protein structure relaxes back to its original shape, shifting the dye back to its non-fluorescent state.
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| [[Image:Principle of HaloTag.jpg]]
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| ==== Mechanism ====
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| ==== Structural highlights ====
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| ==== Relevance ====
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| This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
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| </StructureSection>
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| == References ==
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| <references/>
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