9zr2

Crystal structure of immunity protein SciX from Salmonella Typhimurium

spinqualitylabelsall models 9zr2, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9zr2 is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3ND73_SALTS Publication Abstract from PubMed The type VI secretion system (T6SS) is a dynamic nanomachine used by bacteria to compete for space and nutrients. To kill rival bacteria, the T6SS secretes toxic effector proteins directly into adjacent cells in a contact-dependent manner. Effectors have diverse biochemical functions that arise from subtle structural modifications to related enzyme folds. To protect from self-intoxication, effectors are encoded with a cognate immunity protein in effector-immunity pairs. Immunity proteins inhibit toxicity by directly binding the active site of the toxin and are as structurally diverse as their effectors. Salmonella Typhimurium has several known effectors of diverse biochemical functions, including an orphan toxin-immunity pair Rhs2-SciX. Although Rhs2 has antibacterial activity, the biochemical mechanism of Rhs2 toxicity remains unknown. Here, we take a structural approach and show that Rhs2 is related to BECR family nucleases through modeling and bacterial toxicity assays. Furthermore, we solve an X-ray crystal structure of SciX and study the solution-state properties of the immunity protein to gain insight into the binding and inhibition mechanism of Rhs2. Finally, we discover that Rhs2 binds directly to EF-Tu suggesting that Rhs2 may inhibit translation to cause cell death. Our work probes the structure and function of the orphan toxin-immunity pair Rhs2-SciX, demonstrates the biochemical diversity of Salmonella T6SS effectors, and highlights the conserved T6SS toxicity strategy of binding EF-Tu. Molecular Characterization of the Orphan Toxin-immunity Pair Rhs2-SciX From Salmonella Typhimurium.,Lorente Cobo N, Goyal S, Davidson D, Prehna G J Mol Biol. 2026 Sep 23;438(22):170032. doi: 10.1016/j.jmb.2026.170032. PMID:42778060 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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