2ad9: Difference between revisions
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New page: left|200px<br /> <applet load="2ad9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ad9" /> '''Solution structure of Polypyrimidine Tract ... |
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== | ==Solution structure of Polypyrimidine Tract Binding protein RBD1 complexed with CUCUCU RNA== | ||
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA | <StructureSection load='2ad9' size='340' side='right'caption='[[2ad9]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ad9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AD9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ad9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ad9 OCA], [https://pdbe.org/2ad9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ad9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ad9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ad9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PTBP1_HUMAN PTBP1_HUMAN] Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:11003644</ref> <ref>PMID:15009664</ref> <ref>PMID:16260624</ref> <ref>PMID:21518792</ref> <ref>PMID:16179478</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/2ad9_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ad9 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor. | |||
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.,Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH Science. 2005 Sep 23;309(5743):2054-7. PMID:16179478<ref>PMID:16179478</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2ad9" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Allain | [[Category: Allain FHT]] | ||
[[Category: Auweter | [[Category: Auweter SD]] | ||
[[Category: Black | [[Category: Black DL]] | ||
[[Category: Erat | [[Category: Erat M]] | ||
[[Category: Hargous | [[Category: Hargous Y]] | ||
[[Category: Henning | [[Category: Henning A]] | ||
[[Category: Oberstrass | [[Category: Oberstrass FC]] | ||
[[Category: Pitsch | [[Category: Pitsch S]] | ||
[[Category: Reymond | [[Category: Reymond L]] | ||
[[Category: Wenter | [[Category: Wenter P]] | ||
