21au: Difference between revisions
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==Epitope and functional classification of human neutralizing antibodies against SFTSV Gn== | |||
<StructureSection load='21au' size='340' side='right'caption='[[21au]], [[Resolution|resolution]] 2.69Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[21au]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_fever_with_thrombocytopenia_syndrome_virus Severe fever with thrombocytopenia syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=21AU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=21AU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.69Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=21au FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=21au OCA], [https://pdbe.org/21au PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=21au RCSB], [https://www.ebi.ac.uk/pdbsum/21au PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=21au ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a lethal bunyavirus lacking approved countermeasures. From SFTS survivors, we isolate 84 human monoclonal antibodies (mAbs) against the viral glycoproteins Gn and Gc. Gn-specific mAbs demonstrate superior neutralization breadth and potency compared to the restricted neutralizing activity observed with Gc. Using a high-throughput yeast display deep mutational scanning (DMS) platform, we classify Gn-head mAbs into eight epitope groups, among which four groups (IA, ID, IIIA, and IIIB) confer neutralization. Notably, mAbs BD70-4003 (group IA) and BD70-4017 (group IIIA) exhibit broad neutralization and provide 100% protection in a lethal mouse model. Cryo-electron microscopy (cryo-EM) structural analysis of these mAbs in complex with the Gn head reveals their binding interfaces, directly validating the epitope residues identified by DMS. Our study delineates the antigenic landscape of SFTSV Gn, identifies potent therapeutic candidates, and establishes DMS coupled with structural validation as a powerful framework for antibody discovery against bunyaviruses. | |||
Human monoclonal antibodies that target the SFTSV glycoprotein Gn head from four neutralizing epitope groups.,Wang Q, Li H, Jian F, Han A, Liu Y, Liu J, Yu Y, Wang J, Yu L, Wang Y, Sun H, Ma M, Shao F, Zhu L, Liu W, Cao Y Cell Rep. 2026 Apr 8;45(4):117248. doi: 10.1016/j.celrep.2026.117248. PMID:41955097<ref>PMID:41955097</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 21au" style="background-color:#fffaf0;"></div> | ||
[[Category: Jian | == References == | ||
[[Category: Wang | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Severe fever with thrombocytopenia syndrome virus]] | |||
[[Category: Jian FC]] | |||
[[Category: Wang QR]] | |||
[[Category: Wang YX]] | |||
