22za: Difference between revisions
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Created page with "'''Unreleased structure''' The entry 22za is ON HOLD Authors: Ge, H. Description: crystal structures of RipN from Ralstonia solanacearum Category: Unreleased Structures Category: Ge, H" |
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The | ==crystal structures of RipN from Ralstonia solanacearum== | ||
<StructureSection load='22za' size='340' side='right'caption='[[22za]], [[Resolution|resolution]] 2.28Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[22za]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum_GMI1000 Ralstonia solanacearum GMI1000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=22ZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=22ZA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.278Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=22za FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=22za OCA], [https://pdbe.org/22za PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=22za RCSB], [https://www.ebi.ac.uk/pdbsum/22za PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=22za ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The type III secreted effector RipN from Ralstonia solanacearum is a Nudix hydrolase that suppresses plant immunity by targeting host dinucleotide metabolism. Although RipN preferentially hydrolyzes NADH, the structural basis of its substrate specificity and catalytic mechanism has remained unclear. Here, we report the crystal structures of the full-length RipN and a truncated mutant, RipN(E220A)DeltaN81. RipN adopts a Nudix fold featuring a composite substrate-binding pocket formed by the conserved catalytic core and surrounding structural elements. CASTp analysis indicates that this cavity is well suited to accommodate bulky dinucleotide substrates. Molecular docking analyses reveal that NADH, ADP-ribose and FAD share a conserved binding mode centered on the adenosyl diphosphate scaffold, which is coordinated indirectly through Mg(2+) ions. We further identify Tyr318 and Glu384 as key substrate-discriminating residues that mediate base-specific interactions and steric exclusion, enabling RipN to efficiently hydrolyze NADH while excluding closely related metabolites such as NADPH and UDP-glucose. Based on structural and docking data, we propose a Glu220-dependent, Mg(2+)-assisted catalytic mechanism involving activation of a conserved water molecule for phosphoanhydride bond cleavage. Together, these findings provide mechanistic insight into how RipN selectively targets host dinucleotide metabolites and illustrate how a conserved Nudix scaffold is adapted for effector-specific functions. | |||
Structural basis of dinucleotide substrate recognition and catalysis by the Nudix effector RipN from Ralstonia solanacearum.,Chen X, Zhou Z, Lu L, Xiao C, Cao L, Cao Y, Ge H, Wang W, Gao J Biochem Biophys Res Commun. 2026 Apr 9;808:153433. doi: , 10.1016/j.bbrc.2026.153433. Epub 2026 Feb 11. PMID:41702187<ref>PMID:41702187</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Ge | <div class="pdbe-citations 22za" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Ralstonia solanacearum GMI1000]] | |||
[[Category: Ge H]] | |||
Latest revision as of 08:53, 11 March 2026
crystal structures of RipN from Ralstonia solanacearum
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