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[[Image:1xef.gif|left|200px]]
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{{STRUCTURE_1xef|  PDB=1xef  |  SCENE=  }}
'''Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD'''


==Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD==
<StructureSection load='1xef' size='340' side='right'caption='[[1xef]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xef]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XEF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xef OCA], [https://pdbe.org/1xef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xef RCSB], [https://www.ebi.ac.uk/pdbsum/1xef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xef ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HLYBP_ECOLX HLYBP_ECOLX] Part of the ABC transporter complex HlyBD involved in hemolysin export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/1xef_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xef ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ABC transporter HlyB is a central element of the HlyA secretion machinery, a paradigm of Type I secretion. Here, we describe the crystal structure of the HlyB-NBD (nucleotide-binding domain) with H662 replaced by Ala in complex with ATP/Mg2+. The dimer shows a composite architecture, in which two intact ATP molecules are bound at the interface of the Walker A motif and the C-loop, provided by the two monomers. ATPase measurements confirm that H662 is essential for activity. Based on these data, we propose a model in which E631 and H662, highly conserved among ABC transporters, form a catalytic dyad. Here, H662 acts as a 'linchpin', holding together all required parts of a complicated network of interactions between ATP, water molecules, Mg2+, and amino acids both in cis and trans, necessary for intermonomer communication. Based on biochemical experiments, we discuss the hypothesis that substrate-assisted catalysis, rather than general base catalysis might operate in ABC-ATPases.


==Overview==
H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB.,Zaitseva J, Jenewein S, Jumpertz T, Holland IB, Schmitt L EMBO J. 2005 Jun 1;24(11):1901-10. Epub 2005 May 12. PMID:15889153<ref>PMID:15889153</ref>
The ABC transporter HlyB is a central element of the HlyA secretion machinery, a paradigm of Type I secretion. Here, we describe the crystal structure of the HlyB-NBD (nucleotide-binding domain) with H662 replaced by Ala in complex with ATP/Mg2+. The dimer shows a composite architecture, in which two intact ATP molecules are bound at the interface of the Walker A motif and the C-loop, provided by the two monomers. ATPase measurements confirm that H662 is essential for activity. Based on these data, we propose a model in which E631 and H662, highly conserved among ABC transporters, form a catalytic dyad. Here, H662 acts as a 'linchpin', holding together all required parts of a complicated network of interactions between ATP, water molecules, Mg2+, and amino acids both in cis and trans, necessary for intermonomer communication. Based on biochemical experiments, we discuss the hypothesis that substrate-assisted catalysis, rather than general base catalysis might operate in ABC-ATPases.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1XEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XEF OCA].
</div>
<div class="pdbe-citations 1xef" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB., Zaitseva J, Jenewein S, Jumpertz T, Holland IB, Schmitt L, EMBO J. 2005 Jun 1;24(11):1901-10. Epub 2005 May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15889153 15889153]
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Holland, I B.]]
[[Category: Holland IB]]
[[Category: Jenewein, S.]]
[[Category: Jenewein S]]
[[Category: Schmitt, L.]]
[[Category: Schmitt L]]
[[Category: Zaitseva, J.]]
[[Category: Zaitseva J]]
[[Category: Abc-transporter]]
[[Category: Atp-dependent transport protein]]
[[Category: Atpase]]
[[Category: Haemolysin b]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:55:20 2008''

Latest revision as of 08:06, 25 October 2023

Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD

1xef, resolution 2.50Å

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