24bg: Difference between revisions

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Created page with "'''Unreleased structure''' The entry 24bg is ON HOLD Authors: Liu, C., Li, J., Xiao, H.L., Chen, X. Description: Crystal structure of MonCI mutant N278R-S421A-Q441P Category: Unreleased Structures Category: Chen, X Category: Xiao, H.L Category: Li, J Category: Liu, C"
 
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'''Unreleased structure'''


The entry 24bg is ON HOLD
==Crystal structure of MonCI mutant N278R-S421A-Q441P==
<StructureSection load='24bg' size='340' side='right'caption='[[24bg]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[24bg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_virginiae Streptomyces virginiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=24BG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=24BG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=24bg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=24bg OCA], [https://pdbe.org/24bg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=24bg RCSB], [https://www.ebi.ac.uk/pdbsum/24bg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=24bg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q846W9_STRVG Q846W9_STRVG]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Improving enzyme stability without compromising catalytic activity remains a major challenge in protein engineering. Here, we present a co-evolution-guided strategy to enhance both thermostability and catalytic performance of the flavin-dependent monooxygenase MonCI, an enzyme involved in monensin biosynthesis. By combining sequence covariation analysis with structural filtering, a focused library of 15 single mutants yielded 4 variants with increased stability and activity. Combinatorial assembly generated triple, quadruple and quintuple mutants, with the best-performing quadruple variants exhibiting up to a 10 degrees C increase in melting temperature, a 2.3-fold increase in specific activity, and a 2.1-fold longer half-life, accompanied by enhanced turnover despite reduced substrate affinity. Crystal structures and molecular dynamics simulations reveal that stabilization arises from strengthened intramolecular networks of hydrogen bonds, salt bridges, and hydrophobic interactions, while epistatic effects limit additive improvements. This work provides mechanistic insight into how co-evolving residues modulate enzyme structure and function, presents a useful co-evolution-guided strategy for enzyme design, and advances MonCI as a promising biocatalyst for asymmetric epoxidation.


Authors: Liu, C., Li, J., Xiao, H.L., Chen, X.
Co-evolution-guided engineering of monensin biosynthetic monooxygenase MonCI reveals mechanistic basis for concurrent stability and catalytic enhancement.,Xiao H, Li J, Zhou J, Liu C, Deng Y, Wang S, Tong Z, Liu J, Zheng Z, Zhong J, Li H, Chen X Int J Biol Macromol. 2026 Aug 20;381(Pt 1):154119. doi: , 10.1016/j.ijbiomac.2026.154119. PMID:42624264<ref>PMID:42624264</ref>


Description: Crystal structure of MonCI mutant N278R-S421A-Q441P
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Chen, X]]
<div class="pdbe-citations 24bg" style="background-color:#fffaf0;"></div>
[[Category: Xiao, H.L]]
== References ==
[[Category: Li, J]]
<references/>
[[Category: Liu, C]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces virginiae]]
[[Category: Chen X]]
[[Category: Li J]]
[[Category: Liu C]]
[[Category: Xiao HL]]
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