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New page: left|200px<br /> <applet load="2atx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2atx, resolution 2.65Å" /> '''Crystal Structure o...
 
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[[Image:2atx.gif|left|200px]]<br />
<applet load="2atx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2atx, resolution 2.65&Aring;" />
'''Crystal Structure of the TC10 GppNHp complex'''<br />


==Overview==
==Crystal Structure of the TC10 GppNHp complex==
The specific and rapid formation of protein complexes is essential for, diverse cellular processes such as remodeling of actin filaments in, response to the interaction between Rho GTPases and the Wiskott-Aldrich, syndrome proteins (WASp and N-WASp). Although Cdc42, TC10, and other, members of the Rho family have been implicated in binding to and, activating the WAS proteins, the exact nature of such a protein-protein, recognition process has remained obscure. Here, we describe a mechanism, that ensures rapid and selective long-range Cdc42-WASp recognition. The, crystal structure of TC10, together with mutational and bioinformatic, analyses, proved that the basic region of WASp and two unique glutamates, in Cdc42 generate favorable electrostatic steering forces that control the, accelerated WASp-Cdc42 association reaction. This process is a, prerequisite for WASp activation and a critical step in temporal, regulation and integration of WASp-mediated cellular responses.
<StructureSection load='2atx' size='340' side='right'caption='[[2atx]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2atx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ATX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2atx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2atx OCA], [https://pdbe.org/2atx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2atx RCSB], [https://www.ebi.ac.uk/pdbsum/2atx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2atx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RHOQ_HUMAN RHOQ_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.<ref>PMID:15546864</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/at/2atx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2atx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2ATX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ATX OCA].
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 
== References ==
==Reference==
<references/>
An electrostatic steering mechanism of Cdc42 recognition by Wiskott-Aldrich syndrome proteins., Hemsath L, Dvorsky R, Fiegen D, Carlier MF, Ahmadian MR, Mol Cell. 2005 Oct 28;20(2):313-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16246732 16246732]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Small monomeric GTPase]]
[[Category: Ahmadian MR]]
[[Category: Ahmadian, M.R.]]
[[Category: Carlier MF]]
[[Category: Carlier, M.F.]]
[[Category: Dvorsky R]]
[[Category: Dvorsky, R.]]
[[Category: Fiegen D]]
[[Category: Fiegen, D.]]
[[Category: Hemsath L]]
[[Category: Hemsath, L.]]
[[Category: GNP]]
[[Category: MG]]
[[Category: alpha-beta]]
[[Category: gtpase]]
[[Category: p-loop]]
[[Category: tc10]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:54:45 2007''

Latest revision as of 13:42, 13 March 2024

Crystal Structure of the TC10 GppNHp complex

2atx, resolution 2.65Å

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