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'''Unreleased structure'''


The entry 9wxb is ON HOLD  until 2028-03-25
==Cryo-EM structure of reduced form of formatedehydrogenase from Rhodobacter aestuarii (RaFDH) with NADH==
<StructureSection load='9wxb' size='340' side='right'caption='[[9wxb]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wxb]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_aestuarii Rhodobacter aestuarii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WXB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wxb OCA], [https://pdbe.org/9wxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wxb RCSB], [https://www.ebi.ac.uk/pdbsum/9wxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wxb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1N7KD80_9RHOB A0A1N7KD80_9RHOB]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multi-subunit formate dehydrogenases (FDHs), which catalyze the interconversion of formate and carbon dioxide (CO(2)), have drawn increasing attention for mitigating climate change and advancing environmental protection owing to their advantages of oxygen tolerance and easy heterogenous expression. However, differently sourced multi-subunit FDHs exhibit distinct catalytic biases, and the reasons remain unclear. On the basis of the exceptional observation of Rhodobacter aestuarii FDH favoring CO(2) reduction, this study unveiled an oxidation inhibition effect in exclusively NADH/NAD(+)-involved catalysis via kinetics analysis in terms of different redox couples. Substrate truncation positioned Fdhbeta as the predominant subunit. Further studies based on structural and electrochemical insights interpreted that the slow desorption of NADH is the underlying determinant for the apparent catalytic bias. Knowledge-based rational design helped obtain a beneficial variant, RaFDH beta E260Y, with a 10-fold increased catalytic activity in CO(2) reduction, highlighting its potential for CO(2) biotransformation and applications in low-carbon biomanufacturing. Eventually, bioinformatic analysis suggested that the diaphorase-like subunits and the catalysis regulation mechanism may widely exist in living organisms for modulating the redox balance of oxidoreductases, providing new insights into metabolism and catabolism.


Authors: Zhang, K., Zhang, L.
Understanding the Catalytic Determinant role of Diaphorase-Like Subunit in Formate Dehydrogenases via Redox Couples.,Zhang K, Liu W, Su H, Cui H, Wang Y, Zhu Z, You C, Zhang L Adv Sci (Weinh). 2026 May 19:e75764. doi: 10.1002/advs.75764. PMID:42154453<ref>PMID:42154453</ref>


Description: Cryo-EM structure of reduced form of formatedehydrogenase from Rhodobacter aestuarii (RaFDH) with NADH
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Zhang, K]]
<div class="pdbe-citations 9wxb" style="background-color:#fffaf0;"></div>
[[Category: Zhang, L]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodobacter aestuarii]]
[[Category: Zhang K]]
[[Category: Zhang L]]
Retrieved from "https://proteopedia.org/9wxb"