23vw: Difference between revisions
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==Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex== | |||
<StructureSection load='23vw' size='340' side='right'caption='[[23vw]], [[Resolution|resolution]] 2.39Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[23vw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/DNA_molecule DNA molecule] and [https://en.wikipedia.org/wiki/Peptococcaceae Peptococcaceae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=23VW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=23VW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.39Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=23vw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=23vw OCA], [https://pdbe.org/23vw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=23vw RCSB], [https://www.ebi.ac.uk/pdbsum/23vw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=23vw ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CS12M_THEFR CS12M_THEFR] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (Probable) (PubMed:38261981). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids (Probable) (PubMed:38261981). CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (PubMed:38261981). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, 5'-C/TCN-3' in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:38261981). Upon expression in E.coli as a CRISPR locus inhibits plasmid propagation when targeted to regions essential for plasmid propagation (replication origin but not a selectable marker), probably by inhibiting transcription (PubMed:38261981). Cas12m-crRNA binds DNA in a PAM-dependent, crRNA-guided fashion (PubMed:38261981). Upon expression in E.coli as a CRISPR region preferentially binds to its associated crRNA (PubMed:38261981). Probably required for pre-crRNA processing to mature crRNA (Probable) (PubMed:38261981).<ref>PMID:38261981</ref> <ref>PMID:38261981</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: DNA molecule]] | |||
[[Category: Large Structures]] | |||
[[Category: Peptococcaceae]] | |||
[[Category: Morinaga H]] | |||
[[Category: Nureki O]] | |||
[[Category: Omura SN]] | |||
Latest revision as of 16:39, 8 September 2026
Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex
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