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[[Image:1ztb.gif|left|200px]]
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{{STRUCTURE_1ztb|  PDB=1ztb  |  SCENE=  }}
'''Crystal Structure of Chorismate Synthase from Mycobacterium tuberculosis'''


==Crystal Structure of Chorismate Synthase from Mycobacterium tuberculosis==
<StructureSection load='1ztb' size='340' side='right'caption='[[1ztb]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ztb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZTB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ztb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ztb OCA], [https://pdbe.org/1ztb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ztb RCSB], [https://www.ebi.ac.uk/pdbsum/1ztb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ztb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROC_MYCTU AROC_MYCTU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zt/1ztb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ztb ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In bacteria, fungi, plants, and apicomplexan parasites, the aromatics compounds, such as aromatics amino acids, are synthesized through seven enzymes from the shikimate pathway, which are absent in mammals. The absence of this pathway in mammals make them potential targets for development of new therapy against infectious diseases, such as tuberculosis, which is the world's second commonest cause of death from infectious disease. The last enzyme of shikimate pathway is the chorismate synthase (CS), which is responsible for conversion of the 5-enolpyruvylshikimate-3-phosphate to chorismate. Here, we report the crystallographic structure of CS from Mycobacterium tuberculosis (MtCS) at 2.65 A resolution. The MtCS structure is similar to other CS structures, presenting beta-alpha-beta sandwich structural topology, in which each monomer of MtCS consists of a central helical core. The MtCS can be described as a tetramer formed by a dimer of dimers. However, analytical ultracentrifugation studies suggest the MtCS is a dimer with a more asymmetric shape than observed on the crystallographic dimer and the existence of a low equilibrium between dimer and tetramer. Our results suggest that the MtCS oligomerization is concentration dependent and some conformational changes must be involved on that event.


==Overview==
Structure of chorismate synthase from Mycobacterium tuberculosis.,Dias MV, Borges JC, Ely F, Pereira JH, Canduri F, Ramos CH, Frazzon J, Palma MS, Basso LA, Santos DS, de Azevedo WF Jr J Struct Biol. 2006 May;154(2):130-43. Epub 2006 Jan 17. PMID:16459102<ref>PMID:16459102</ref>
In bacteria, fungi, plants, and apicomplexan parasites, the aromatics compounds, such as aromatics amino acids, are synthesized through seven enzymes from the shikimate pathway, which are absent in mammals. The absence of this pathway in mammals make them potential targets for development of new therapy against infectious diseases, such as tuberculosis, which is the world's second commonest cause of death from infectious disease. The last enzyme of shikimate pathway is the chorismate synthase (CS), which is responsible for conversion of the 5-enolpyruvylshikimate-3-phosphate to chorismate. Here, we report the crystallographic structure of CS from Mycobacterium tuberculosis (MtCS) at 2.65 A resolution. The MtCS structure is similar to other CS structures, presenting beta-alpha-beta sandwich structural topology, in which each monomer of MtCS consists of a central helical core. The MtCS can be described as a tetramer formed by a dimer of dimers. However, analytical ultracentrifugation studies suggest the MtCS is a dimer with a more asymmetric shape than observed on the crystallographic dimer and the existence of a low equilibrium between dimer and tetramer. Our results suggest that the MtCS oligomerization is concentration dependent and some conformational changes must be involved on that event.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1ZTB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTB OCA].
</div>
<div class="pdbe-citations 1ztb" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of chorismate synthase from Mycobacterium tuberculosis., Dias MV, Borges JC, Ely F, Pereira JH, Canduri F, Ramos CH, Frazzon J, Palma MS, Basso LA, Santos DS, de Azevedo WF Jr, J Struct Biol. 2006 May;154(2):130-43. Epub 2006 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16459102 16459102]
*[[Chorismate synthase|Chorismate synthase]]
[[Category: Chorismate synthase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Azevedo Jr WF]]
[[Category: Basso, L A.]]
[[Category: Basso LA]]
[[Category: Borges, J C.]]
[[Category: Borges JC]]
[[Category: Canduri, F.]]
[[Category: Canduri F]]
[[Category: Dias, M V.B.]]
[[Category: Dias MVB]]
[[Category: Ely, F.]]
[[Category: Ely F]]
[[Category: Frazzon, J.]]
[[Category: Frazzon J]]
[[Category: Jr., W F.Azevedo.]]
[[Category: Palma MS]]
[[Category: Palma, M S.]]
[[Category: Pereira JH]]
[[Category: Pereira, J H.]]
[[Category: Ramos CHI]]
[[Category: Ramos, C H.I.]]
[[Category: Santos DS]]
[[Category: Santos, D S.]]
[[Category: Beta-alpha-beta]]
[[Category: Flavoprotein]]
[[Category: Shikimate]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:02:46 2008''

Latest revision as of 07:14, 23 August 2023

Crystal Structure of Chorismate Synthase from Mycobacterium tuberculosis

1ztb, resolution 2.65Å

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