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[[Image:2a8d.jpg|left|200px]]
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{{STRUCTURE_2a8d|  PDB=2a8d  |  SCENE=  }}
'''Haemophilus influenzae beta-carbonic anhydrase complexed with bicarbonate'''


==Haemophilus influenzae beta-carbonic anhydrase complexed with bicarbonate==
<StructureSection load='2a8d' size='340' side='right'caption='[[2a8d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2a8d]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8d OCA], [https://pdbe.org/2a8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8d RCSB], [https://www.ebi.ac.uk/pdbsum/2a8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAN_HAEIN CAN_HAEIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8d ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of orthologues from Porphyridium purpureum, Escherichia coli, and Mycobacterium tuberculosis. Here we present the structure of an additional member of that subclass, that from Haemophilus influenzae, as well as detailed kinetic analysis, revealing the correspondence between structural classification and kinetic profile for this subclass. In addition, we identify a unique, noncatalytic binding mode for the substrate bicarbonate that occurs in both the H. influenzae and E. coli enzymes. The kinetic and structural analysis indicates that binding of bicarbonate in this site of the enzyme may modulate its activity by influencing a pH-dependent, cooperative transition between active and inactive forms. We hypothesize that the two structural subclasses of beta-CAs may provide models for the proposed active and inactive forms of the H. influenzae and E. coli enzymes.


==Overview==
Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase.,Cronk JD, Rowlett RS, Zhang KY, Tu C, Endrizzi JA, Lee J, Gareiss PC, Preiss JR Biochemistry. 2006 Apr 11;45(14):4351-61. PMID:16584170<ref>PMID:16584170</ref>
The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of orthologues from Porphyridium purpureum, Escherichia coli, and Mycobacterium tuberculosis. Here we present the structure of an additional member of that subclass, that from Haemophilus influenzae, as well as detailed kinetic analysis, revealing the correspondence between structural classification and kinetic profile for this subclass. In addition, we identify a unique, noncatalytic binding mode for the substrate bicarbonate that occurs in both the H. influenzae and E. coli enzymes. The kinetic and structural analysis indicates that binding of bicarbonate in this site of the enzyme may modulate its activity by influencing a pH-dependent, cooperative transition between active and inactive forms. We hypothesize that the two structural subclasses of beta-CAs may provide models for the proposed active and inactive forms of the H. influenzae and E. coli enzymes.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2A8D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8D OCA].
</div>
<div class="pdbe-citations 2a8d" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase., Cronk JD, Rowlett RS, Zhang KY, Tu C, Endrizzi JA, Lee J, Gareiss PC, Preiss JR, Biochemistry. 2006 Apr 11;45(14):4351-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16584170 16584170]
*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
[[Category: Carbonate dehydratase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cronk, J D.]]
[[Category: Cronk JD]]
[[Category: Endrizzi, J A.]]
[[Category: Endrizzi JA]]
[[Category: Gareiss, P C.]]
[[Category: Gareiss PC]]
[[Category: Lee, J.]]
[[Category: Lee J]]
[[Category: Preiss, J R.]]
[[Category: Preiss JR]]
[[Category: Rowlett, R S.]]
[[Category: Rowlett RS]]
[[Category: Tu, C.]]
[[Category: Tu C]]
[[Category: Zhang, K Y.J.]]
[[Category: Zhang KYJ]]
[[Category: Carbonic anhydrase]]
[[Category: Protein-bicarbonate complex]]
[[Category: X-ray structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:44:22 2008''

Latest revision as of 07:20, 23 August 2023

Haemophilus influenzae beta-carbonic anhydrase complexed with bicarbonate

2a8d, resolution 2.20Å

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