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[[Image:2aud.gif|left|200px]]
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{{STRUCTURE_2aud|  PDB=2aud  |  SCENE=  }}
'''Unliganded HincII'''


==Unliganded HincII==
<StructureSection load='2aud' size='340' side='right'caption='[[2aud]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2aud]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aud OCA], [https://pdbe.org/2aud PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aud RCSB], [https://www.ebi.ac.uk/pdbsum/2aud PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aud ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2C2_HAEIF T2C2_HAEIF] Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 2.1A crystal structure of the unliganded type II restriction endonuclease, HincII, is described. Although the asymmetric unit contains only a single monomer, crystal lattice contacts bring two monomers together to form a dimer very similar to that found in the DNA bound form. Comparison with the published DNA bound structure reveals that the DNA binding pocket is expanded in the unliganded structure. Comparison of the unliganded and DNA liganded structures reveals a simple rotation of subunits by 11 degrees each, or 22 degrees total, to a more closed structure around the bound DNA. Comparison of this conformational change to that observed in the other type II restriction endonucleases where DNA bound and unliganded forms have both been characterized, shows considerable variation in the types of conformational changes that can occur. The conformational changes in three can be described by a simple rotation of subunits, while in two others both rotation and translation of subunits relative to one another occurs. In addition, the endonucleases having subunits that undergo the greatest amount of rotation upon DNA binding are found to be those that distort the bound DNA the least, suggesting that DNA bending may be less facile in dimers possessing greater flexibility.


==Overview==
DNA-induced conformational changes in type II restriction endonucleases: the structure of unliganded HincII.,Little EJ, Horton NC J Mol Biol. 2005 Aug 5;351(1):76-88. PMID:15993893<ref>PMID:15993893</ref>
The 2.1A crystal structure of the unliganded type II restriction endonuclease, HincII, is described. Although the asymmetric unit contains only a single monomer, crystal lattice contacts bring two monomers together to form a dimer very similar to that found in the DNA bound form. Comparison with the published DNA bound structure reveals that the DNA binding pocket is expanded in the unliganded structure. Comparison of the unliganded and DNA liganded structures reveals a simple rotation of subunits by 11 degrees each, or 22 degrees total, to a more closed structure around the bound DNA. Comparison of this conformational change to that observed in the other type II restriction endonucleases where DNA bound and unliganded forms have both been characterized, shows considerable variation in the types of conformational changes that can occur. The conformational changes in three can be described by a simple rotation of subunits, while in two others both rotation and translation of subunits relative to one another occurs. In addition, the endonucleases having subunits that undergo the greatest amount of rotation upon DNA binding are found to be those that distort the bound DNA the least, suggesting that DNA bending may be less facile in dimers possessing greater flexibility.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2AUD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUD OCA].
</div>
<div class="pdbe-citations 2aud" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
DNA-induced conformational changes in type II restriction endonucleases: the structure of unliganded HincII., Little EJ, Horton NC, J Mol Biol. 2005 Aug 5;351(1):76-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15993893 15993893]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Horton NC]]
[[Category: Horton, N C.]]
[[Category: Little EJ]]
[[Category: Little, E J.]]
[[Category: Blunt cutter]]
[[Category: Dna binding]]
[[Category: Restriction endonuclease]]
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Retrieved from "https://proteopedia.org/2aud"