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New page: left|200px<br /> <applet load="2fi2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fi2" /> '''Solution structure of the SCAN homodimer fr...
 
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[[Image:2fi2.gif|left|200px]]<br />
<applet load="2fi2" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2fi2" />
'''Solution structure of the SCAN homodimer from MZF-1/ZNF42'''<br />


==Overview==
==Solution structure of the SCAN homodimer from MZF-1/ZNF42==
The SCAN domain mediates interactions between members of a subfamily of, zinc-finger transcription factors and is found in more than 60 C2H2 zinc, finger genes in the human genome, including the tumor suppressor gene, myeloid zinc finger 1 (MZF1). Glutathione-S-transferase pull-down assays, showed that the MZF1 SCAN domain self-associates, and a Kd value of 600 nM, was measured by intrinsic tryptophan fluorescence polarization. The MZF1, structure determined by NMR spectroscopy revealed a domain-swapped dimer., Each monomer consists of five alpha helices in two subdomains connected by, the alpha2-alpha3 loop. Residues from helix 3 of each monomer compose the, core of the dimer interface, while the alpha1-alpha2 loop and helix 2 pack, against helices 3 and 5 from the opposing monomer. Comprehensive sequence, analysis is coupled with the first high-resolution structure of a SCAN, dimer to provide an initial view of the recognition elements that govern, dimerization for this large family of transcription factors.
<StructureSection load='2fi2' size='340' side='right'caption='[[2fi2]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2fi2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FI2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fi2 OCA], [https://pdbe.org/2fi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fi2 RCSB], [https://www.ebi.ac.uk/pdbsum/2fi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fi2 ProSAT], [https://www.topsan.org/Proteins/CESG/2fi2 TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MZF1_HUMAN MZF1_HUMAN] Binds to target promoter DNA and functions as trancription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development.<ref>PMID:15541732</ref> <ref>PMID:17851584</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/2fi2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fi2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The SCAN domain mediates interactions between members of a subfamily of zinc-finger transcription factors and is found in more than 60 C2H2 zinc finger genes in the human genome, including the tumor suppressor gene myeloid zinc finger 1 (MZF1). Glutathione-S-transferase pull-down assays showed that the MZF1 SCAN domain self-associates, and a Kd value of 600 nM was measured by intrinsic tryptophan fluorescence polarization. The MZF1 structure determined by NMR spectroscopy revealed a domain-swapped dimer. Each monomer consists of five alpha helices in two subdomains connected by the alpha2-alpha3 loop. Residues from helix 3 of each monomer compose the core of the dimer interface, while the alpha1-alpha2 loop and helix 2 pack against helices 3 and 5 from the opposing monomer. Comprehensive sequence analysis is coupled with the first high-resolution structure of a SCAN dimer to provide an initial view of the recognition elements that govern dimerization for this large family of transcription factors.


==Disease==
Structure of the SCAN domain from the tumor suppressor protein MZF1.,Peterson FC, Hayes PL, Waltner JK, Heisner AK, Jensen DR, Sander TL, Volkman BF J Mol Biol. 2006 Oct 13;363(1):137-47. Epub 2006 Jul 31. PMID:16950398<ref>PMID:16950398</ref>
Known disease associated with this structure: Mental retardation syndrome, X-linked, Siderius type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=300560 300560]]


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2FI2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FI2 OCA].
</div>
 
<div class="pdbe-citations 2fi2" style="background-color:#fffaf0;"></div>
==Reference==
== References ==
Structure of the SCAN domain from the tumor suppressor protein MZF1., Peterson FC, Hayes PL, Waltner JK, Heisner AK, Jensen DR, Sander TL, Volkman BF, J Mol Biol. 2006 Oct 13;363(1):137-47. Epub 2006 Jul 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16950398 16950398]
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]
[[Category: Peterson FC]]
[[Category: Peterson, F.C.]]
[[Category: Sander TL]]
[[Category: Sander, T.L.]]
[[Category: Volkman BF]]
[[Category: Volkman, B.F.]]
[[Category: Waltner JK]]
[[Category: Waltner, J.K.]]
[[Category: center for eukaryotic structural genomics]]
[[Category: cesg]]
[[Category: homodimer]]
[[Category: mzf-1]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: scan domain]]
[[Category: structural genomics]]
[[Category: transcription factor]]
[[Category: znf-42]]
 
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Retrieved from "https://proteopedia.org/2fi2"