2c7s: Difference between revisions

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[[Image:2c7s.gif|left|200px]]
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{{STRUCTURE_2c7s|  PDB=2c7s  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF HUMAN PROTEIN TYROSINE PHOSPHATASE KAPPA AT 1.95A RESOLUTION'''


==Crystal structure of human protein tyrosine phosphatase kappa at 1.95A resolution==
<StructureSection load='2c7s' size='340' side='right'caption='[[2c7s]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2c7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C7S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7s OCA], [https://pdbe.org/2c7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c7s RCSB], [https://www.ebi.ac.uk/pdbsum/2c7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c7s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PTPRK_HUMAN PTPRK_HUMAN] Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.<ref>PMID:19836242</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/2c7s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c7s ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The receptor-type protein tyrosine phosphatases (RPTPs) are integral membrane proteins composed of extracellular adhesion molecule-like domains, a single transmembrane domain, and a cytoplasmic domain. The cytoplasmic domain consists of tandem PTP domains, of which the D1 domain is enzymatically active. RPTPkappa is a member of the R2A/IIb subfamily of RPTPs along with RPTPmu, RPTPrho, and RPTPlambda. Here, we have determined the crystal structure of catalytically active, monomeric D1 domain of RPTPkappa at 1.9 A. Structural comparison with other PTP family members indicates an overall classical PTP architecture of twisted mixed beta-sheets flanked by alpha-helices, in which the catalytically important WPD loop is in an unhindered open conformation. Though the residues forming the dimeric interface in the RPTPmu structure are all conserved, they are not involved in the protein-protein interaction in RPTPkappa. The N-terminal beta-strand, formed by betax association with betay, is conserved only in RPTPs but not in cytosolic PTPs, and this feature is conserved in the RPTPkappa structure forming a beta-strand. Analytical ultracentrifugation studies show that the presence of reducing agents and higher ionic strength are necessary to maintain RPTPkappa as a monomer. In this family the crystal structure of catalytically active RPTPmu D1 was solved as a dimer, but the dimerization was proposed to be a consequence of crystallization since the protein was monomeric in solution. In agreement, we show that RPTPkappa is monomeric in solution and crystal structure.


==Overview==
The crystal structure of human receptor protein tyrosine phosphatase kappa phosphatase domain 1.,Eswaran J, Debreczeni JE, Longman E, Barr AJ, Knapp S Protein Sci. 2006 Jun;15(6):1500-5. Epub 2006 May 2. PMID:16672235<ref>PMID:16672235</ref>
The receptor-type protein tyrosine phosphatases (RPTPs) are integral membrane proteins composed of extracellular adhesion molecule-like domains, a single transmembrane domain, and a cytoplasmic domain. The cytoplasmic domain consists of tandem PTP domains, of which the D1 domain is enzymatically active. RPTPkappa is a member of the R2A/IIb subfamily of RPTPs along with RPTPmu, RPTPrho, and RPTPlambda. Here, we have determined the crystal structure of catalytically active, monomeric D1 domain of RPTPkappa at 1.9 A. Structural comparison with other PTP family members indicates an overall classical PTP architecture of twisted mixed beta-sheets flanked by alpha-helices, in which the catalytically important WPD loop is in an unhindered open conformation. Though the residues forming the dimeric interface in the RPTPmu structure are all conserved, they are not involved in the protein-protein interaction in RPTPkappa. The N-terminal beta-strand, formed by betax association with betay, is conserved only in RPTPs but not in cytosolic PTPs, and this feature is conserved in the RPTPkappa structure forming a beta-strand. Analytical ultracentrifugation studies show that the presence of reducing agents and higher ionic strength are necessary to maintain RPTPkappa as a monomer. In this family the crystal structure of catalytically active RPTPmu D1 was solved as a dimer, but the dimerization was proposed to be a consequence of crystallization since the protein was monomeric in solution. In agreement, we show that RPTPkappa is monomeric in solution and crystal structure.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2C7S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7S OCA].
</div>
<div class="pdbe-citations 2c7s" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
The crystal structure of human receptor protein tyrosine phosphatase kappa phosphatase domain 1., Eswaran J, Debreczeni JE, Longman E, Barr AJ, Knapp S, Protein Sci. 2006 Jun;15(6):1500-5. Epub 2006 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16672235 16672235]
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein-tyrosine-phosphatase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Arrowsmith C]]
[[Category: Arrowsmith, C.]]
[[Category: Barr A]]
[[Category: Barr, A.]]
[[Category: Burgess N]]
[[Category: Burgess, N.]]
[[Category: Das S]]
[[Category: Das, S.]]
[[Category: Debreczeni JE]]
[[Category: Debreczeni, J E.]]
[[Category: Edwards A]]
[[Category: Delft, F Von.]]
[[Category: Eswaran J]]
[[Category: Edwards, A.]]
[[Category: Gileadi O]]
[[Category: Eswaran, J.]]
[[Category: Knapp S]]
[[Category: Gileadi, O.]]
[[Category: Longman E]]
[[Category: Knapp, S.]]
[[Category: Sundstron M]]
[[Category: Longman, E.]]
[[Category: Ugochukwu E]]
[[Category: Sundstron, M.]]
[[Category: Weigelt J]]
[[Category: Ugochukwu, E.]]
[[Category: Von Delft F]]
[[Category: Weigelt, J.]]
[[Category: Glycoprotein]]
[[Category: Hydrolase]]
[[Category: Immunoglobulin domain]]
[[Category: Protein phosphatase]]
[[Category: Ptprk]]
[[Category: Receptor]]
[[Category: Receptor type tyrosine phosphatase kappa]]
[[Category: Transmembrane]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:24:05 2008''
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