2d49: Difference between revisions

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[[Image:2d49.gif|left|200px]]
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{{STRUCTURE_2d49|  PDB=2d49  |  SCENE=  }}
'''Solution structure of the Chitin-Binding Domain of Streptomyces griseus Chitinase C'''


==Solution structure of the Chitin-Binding Domain of Streptomyces griseus Chitinase C==
<StructureSection load='2d49' size='340' side='right'caption='[[2d49]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2d49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D49 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 30 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d49 OCA], [https://pdbe.org/2d49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d49 RCSB], [https://www.ebi.ac.uk/pdbsum/2d49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d49 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O50152_STRGR O50152_STRGR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d4/2d49_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d49 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chitinase C from Streptomyces griseus HUT6037 was discovered as the first bacterial chitinase in family 19 other than chitinases found in higher plants. Chitinase C comprises two domains: a chitin-binding domain (ChBD(ChiC)) for attachment to chitin and a chitin-catalytic domain for digesting chitin. The structure of ChBD(ChiC) was determined by means of 13C-, 15N-, and 1H-resonance nuclear magnetic resonance (NMR) spectroscopy. The conformation of its backbone comprised two beta-sheets composed of two and three antiparallel beta-strands, respectively, this being very similar to the backbone conformations of the cellulose-binding domain of endoglucanase Z from Erwinia chrysanthemi (CBD(EGZ)) and the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12 (ChBD(ChiA1)). The interaction between ChBD(ChiC) and hexa-N-acetyl-chitohexaose was monitored through chemical shift perturbations, which showed that ChBD(ChiC) interacted with the substrate through two aromatic rings exposed to the solvent as CBD(EGZ) interacts with cellulose through three characteristic aromatic rings. Comparison of the conformations of ChBD(ChiA1), ChBD(ChiC), and other typical chitin- and cellulose-binding domains, which have three solvent-exposed aromatic residues responsible for binding to polysaccharides, has suggested that they have adopted versatile binding site conformations depending on the substrates, with almost the same backbone conformations being retained.


==Overview==
Identification of the substrate interaction region of the chitin-binding domain of Streptomyces griseus chitinase C.,Akagi K, Watanabe J, Hara M, Kezuka Y, Chikaishi E, Yamaguchi T, Akutsu H, Nonaka T, Watanabe T, Ikegami T J Biochem. 2006 Mar;139(3):483-93. PMID:16567413<ref>PMID:16567413</ref>
Chitinase C from Streptomyces griseus HUT6037 was discovered as the first bacterial chitinase in family 19 other than chitinases found in higher plants. Chitinase C comprises two domains: a chitin-binding domain (ChBD(ChiC)) for attachment to chitin and a chitin-catalytic domain for digesting chitin. The structure of ChBD(ChiC) was determined by means of 13C-, 15N-, and 1H-resonance nuclear magnetic resonance (NMR) spectroscopy. The conformation of its backbone comprised two beta-sheets composed of two and three antiparallel beta-strands, respectively, this being very similar to the backbone conformations of the cellulose-binding domain of endoglucanase Z from Erwinia chrysanthemi (CBD(EGZ)) and the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12 (ChBD(ChiA1)). The interaction between ChBD(ChiC) and hexa-N-acetyl-chitohexaose was monitored through chemical shift perturbations, which showed that ChBD(ChiC) interacted with the substrate through two aromatic rings exposed to the solvent as CBD(EGZ) interacts with cellulose through three characteristic aromatic rings. Comparison of the conformations of ChBD(ChiA1), ChBD(ChiC), and other typical chitin- and cellulose-binding domains, which have three solvent-exposed aromatic residues responsible for binding to polysaccharides, has suggested that they have adopted versatile binding site conformations depending on the substrates, with almost the same backbone conformations being retained.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2D49 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D49 OCA].
</div>
<div class="pdbe-citations 2d49" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Identification of the substrate interaction region of the chitin-binding domain of Streptomyces griseus chitinase C., Akagi K, Watanabe J, Hara M, Kezuka Y, Chikaishi E, Yamaguchi T, Akutsu H, Nonaka T, Watanabe T, Ikegami T, J Biochem. 2006 Mar;139(3):483-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16567413 16567413]
*[[Chitinase 3D structures|Chitinase 3D structures]]
[[Category: Chitinase]]
== References ==
[[Category: Single protein]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces griseus]]
[[Category: Streptomyces griseus]]
[[Category: Akagi, K.]]
[[Category: Akagi K]]
[[Category: Akutsu, H.]]
[[Category: Akutsu H]]
[[Category: Chikaishi, E.]]
[[Category: Chikaishi E]]
[[Category: Hara, M.]]
[[Category: Hara M]]
[[Category: Ikegami, T.]]
[[Category: Ikegami T]]
[[Category: Kezuka, Y.]]
[[Category: Kezuka Y]]
[[Category: Nonaka, T.]]
[[Category: Nonaka T]]
[[Category: Watanabe, J.]]
[[Category: Watanabe J]]
[[Category: Watanabe, T.]]
[[Category: Watanabe T]]
[[Category: Yamaguchi, T.]]
[[Category: Yamaguchi T]]
[[Category: Chbd]]
[[Category: Chitin binding domain]]
[[Category: Chitinase]]
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Retrieved from "https://proteopedia.org/2d49"