2e7p: Difference between revisions

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[[Image:2e7p.jpg|left|200px]]


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==Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides==
The line below this paragraph, containing "STRUCTURE_2e7p", creates the "Structure Box" on the page.
<StructureSection load='2e7p' size='340' side='right'caption='[[2e7p]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2e7p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_tremuloides Populus tremula x Populus tremuloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E7P FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
{{STRUCTURE_2e7p| PDB=2e7p  | SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e7p OCA], [https://pdbe.org/2e7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e7p RCSB], [https://www.ebi.ac.uk/pdbsum/2e7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e7p ProSAT]</span></td></tr>
 
</table>
'''Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q5PSJ1_POPPZ Q5PSJ1_POPPZ]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/2e7p_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e7p ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.
When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.


==About this Structure==
Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1.,Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP Proc Natl Acad Sci U S A. 2007 May 1;104(18):7379-84. Epub 2007 Apr 25. PMID:17460036<ref>PMID:17460036</ref>
2E7P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_tremuloides Populus tremula x populus tremuloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7P OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1., Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP, Proc Natl Acad Sci U S A. 2007 May 1;104(18):7379-84. Epub 2007 Apr 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17460036 17460036]
</div>
[[Category: Populus tremula x populus tremuloides]]
<div class="pdbe-citations 2e7p" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Aimoto, S.]]
<references/>
[[Category: Hase, T.]]
__TOC__
[[Category: Jacquot, J P.]]
</StructureSection>
[[Category: Kawakami, T.]]
[[Category: Large Structures]]
[[Category: Kusunoki, M.]]
[[Category: Populus tremula x Populus tremuloides]]
[[Category: Rouhier, N.]]
[[Category: Aimoto S]]
[[Category: Takahashi, T.]]
[[Category: Hase T]]
[[Category: Unno, H.]]
[[Category: Jacquot JP]]
[[Category: Electron transport]]
[[Category: Kawakami T]]
[[Category: Glutaredoxin]]
[[Category: Kusunoki M]]
[[Category: Poplar]]
[[Category: Rouhier N]]
[[Category: Thioredoxin fold]]
[[Category: Takahashi T]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:05:22 2008''
[[Category: Unno H]]
Retrieved from "https://proteopedia.org/2e7p"