2ek9: Difference between revisions

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[[Image:2ek9.jpg|left|200px]]


<!--
==Aminopeptidase from Aneurinibacillus sp. strain AM-1 with Bestatin==
The line below this paragraph, containing "STRUCTURE_2ek9", creates the "Structure Box" on the page.
<StructureSection load='2ek9' size='340' side='right'caption='[[2ek9]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2ek9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aneurinibacillus_sp._AM-1 Aneurinibacillus sp. AM-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EK9 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BES:2-(3-AMINO-2-HYDROXY-4-PHENYL-BUTYRYLAMINO)-4-METHYL-PENTANOIC+ACID'>BES</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_2ek9| PDB=2ek9 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ek9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ek9 OCA], [https://pdbe.org/2ek9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ek9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ek9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ek9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2V759_9BACL A2V759_9BACL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ek/2ek9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ek9 ConSurf].
<div style="clear:both"></div>


'''Aminopeptidase from Aneurinibacillus sp. strain AM-1 with Bestatin'''
==See Also==
 
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination.
[[Category: Aneurinibacillus sp. AM-1]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Akioka M]]
2EK9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK9 OCA].
[[Category: Nakano H]]
 
[[Category: Watanabe K]]
==Reference==
Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1., Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17142913 17142913]
[[Category: Bacteria]]
[[Category: Single protein]]
[[Category: Akioka, M.]]
[[Category: Nakano, H.]]
[[Category: Watanabe, K.]]
[[Category: Hydrolase]]
[[Category: Metalloproteinase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:40:22 2008''

Latest revision as of 08:42, 25 October 2023

Aminopeptidase from Aneurinibacillus sp. strain AM-1 with Bestatin

2ek9, resolution 1.97Å

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