2ig2: Difference between revisions

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[[Image:2ig2.gif|left|200px]]<br />
<applet load="2ig2" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2ig2, resolution 3.0&Aring;" />
'''DIR PRIMAERSTRUKTUR DES KRISTALLISIERBAREN MONOKLONALEN IMMUNOGLOBULINS IGG1 KOL. II. AMINOSAEURESEQUENZ DER L-KETTE, LAMBDA-TYP, SUBGRUPPE I (GERMAN)'''<br />


==Overview==
==DIR PRIMAERSTRUKTUR DES KRISTALLISIERBAREN MONOKLONALEN IMMUNOGLOBULINS IGG1 KOL. II. AMINOSAEURESEQUENZ DER L-KETTE, LAMBDA-TYP, SUBGRUPPE I (GERMAN)==
The immunoglobulin Kol was the first intact antibody molecule which was, characterized by high-resolution X-ray crystallography. Furthermore the, complete amino-acid sequence of the heavy (H)-chain is known. Here we, report the complete amino-acid sequence of the light (L)-chain of the, monoclonal immunoglobulin Kol (IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due to its structure is of the, lambda-type. With the characteristic amino acids threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116, 154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of the same, isotype are known. The amino-acid sequence of the N-terminal variable part, is characteristic of subgroup 1. This contribution completes the primary, structure of IgG1 Kol.
<StructureSection load='2ig2' size='340' side='right'caption='[[2ig2]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ig2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ig2 1ig2]. The April 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nanobodies''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_4 10.2210/rcsb_pdb/mom_2011_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IG2 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ig2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ig2 OCA], [https://pdbe.org/2ig2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ig2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ig2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ig2 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/2ig2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ig2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The immunoglobulin Kol was the first intact antibody molecule which was characterized by high-resolution X-ray crystallography. Furthermore the complete amino-acid sequence of the heavy (H)-chain is known. Here we report the complete amino-acid sequence of the light (L)-chain of the monoclonal immunoglobulin Kol (IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due to its structure is of the lambda-type. With the characteristic amino acids threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116, 154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of the same isotype are known. The amino-acid sequence of the N-terminal variable part is characteristic of subgroup 1. This contribution completes the primary structure of IgG1 Kol.


==About this Structure==
[The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I],Kratzin HD, Palm W, Stangel M, Schmidt WE, Friedrich J, Hilschmann N Biol Chem Hoppe Seyler. 1989 Mar;370(3):263-72. PMID:2713105<ref>PMID:2713105</ref>
2IG2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure superseeds the now removed PDB entry 1IG2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IG2 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I], Kratzin HD, Palm W, Stangel M, Schmidt WE, Friedrich J, Hilschmann N, Biol Chem Hoppe Seyler. 1989 Mar;370(3):263-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2713105 2713105]
</div>
[[Category: Homo sapiens]]
<div class="pdbe-citations 2ig2" style="background-color:#fffaf0;"></div>
[[Category: Protein complex]]
[[Category: Huber, R.]]
[[Category: Marquart, M.]]
[[Category: immunoglobulin]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:44:23 2007''
==See Also==
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Nanobodies]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Huber, R]]
[[Category: Marquart, M]]
[[Category: Immunoglobulin]]

Latest revision as of 20:42, 20 October 2021

DIR PRIMAERSTRUKTUR DES KRISTALLISIERBAREN MONOKLONALEN IMMUNOGLOBULINS IGG1 KOL. II. AMINOSAEURESEQUENZ DER L-KETTE, LAMBDA-TYP, SUBGRUPPE I (GERMAN)

2ig2, resolution 3.00Å

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