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New page: left|200px<br /> <applet load="2ila" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ila, resolution 2.3Å" /> '''STRUCTURE OF INTERLE...
 
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[[Image:2ila.gif|left|200px]]<br />
<applet load="2ila" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2ila, resolution 2.3&Aring;" />
'''STRUCTURE OF INTERLEUKIN 1ALPHA AT 2.7-ANGSTROMS RESOLUTION'''<br />


==Overview==
==STRUCTURE OF INTERLEUKIN 1ALPHA AT 2.7-ANGSTROMS RESOLUTION==
The interleukin 1 (IL-1) family of proteins has a central role in, modulating immune and inflammatory responses. Two major IL-1 proteins, designated alpha (IL-1 alpha) and beta (IL-1 beta), are produced by, activated macrophages and other cell types. In an effort to understand the, similarities and differences in the physicochemical and functional, properties of these two proteins, a program was initiated to determine, their structures. Crystals of IL-1 alpha were grown, and the, three-dimensional structure at 2.7-A resolution was solved. The technique, of multiple-wavelength anomalous dispersion (MAD) with the, selenomethionine form of IL-1 alpha was utilized in combination with a, single mercury derivative to provide the starting phases. Partial, refinement of the IL-1 alpha model has been performed as well. The overall, structure is composed of 14 beta-strands and a 3(10) helix. The core of, this structure is a capped beta-barrell that possesses 3-fold symmetry and, displays a topology similar to that observed for IL-1 beta [Priestle, J., P., et al. (1988) EMBO J. 7, 339-343] and soybean trypsin inhibitor (STI), [McLachlan, A. D. (1979) J. Mol. Biol. 133, 557-563]. In this paper, the, overall structure of IL-1 alpha and the nature and fidelity of the, internal 3-fold symmetry are discussed. Comparisons with IL-1 beta and STI, are made within these contexts.
<StructureSection load='2ila' size='340' side='right'caption='[[2ila]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ila]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ILA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ILA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ila FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ila OCA], [https://pdbe.org/2ila PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ila RCSB], [https://www.ebi.ac.uk/pdbsum/2ila PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ila ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IL1A_HUMAN IL1A_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/il/2ila_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ila ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2ILA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ILA OCA].
*[[Interleukin 3D structures|Interleukin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of interleukin 1 alpha at 2.7-A resolution., Graves BJ, Hatada MH, Hendrickson WA, Miller JK, Madison VS, Satow Y, Biochemistry. 1990 Mar 20;29(11):2679-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2346741 2346741]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Graves, B.J.]]
[[Category: Graves BJ]]
[[Category: Hatada, M.H.]]
[[Category: Hatada MH]]
[[Category: cytokine]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:45:38 2007''

Latest revision as of 09:03, 21 February 2024

STRUCTURE OF INTERLEUKIN 1ALPHA AT 2.7-ANGSTROMS RESOLUTION

2ila, resolution 2.30Å

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