2fa1: Difference between revisions

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[[Image:2fa1.gif|left|200px]]


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==Crystal structure of PhnF C-terminal domain==
The line below this paragraph, containing "STRUCTURE_2fa1", creates the "Structure Box" on the page.
<StructureSection load='2fa1' size='340' side='right'caption='[[2fa1]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2fa1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FA1 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene></td></tr>
{{STRUCTURE_2fa1|  PDB=2fa1  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fa1 OCA], [https://pdbe.org/2fa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fa1 RCSB], [https://www.ebi.ac.uk/pdbsum/2fa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fa1 ProSAT], [https://www.topsan.org/Proteins/MCSG/2fa1 TOPSAN]</span></td></tr>
 
</table>
'''Crystal structure of PhnF C-terminal domain'''
== Function ==
 
[https://www.uniprot.org/uniprot/PHNF_ECOLI PHNF_ECOLI] Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known. By similarity could be a transcriptional regulator.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 A resolution by the single wavelength anomalous dispersion (SAD) method. The PhnF protein belongs to the HutC subfamily of the large GntR transcriptional regulator family. Members of this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according to their heterogenic C-terminal domains, which are involved in effector binding and oligomerization. The C-PhnF structure provides for the first time the scaffold of this domain for the HutC subfamily, which covers about 31% of GntR-like regulators. The structure represents a mixture of alpha-helices and beta-strands, with a six-stranded antiparallel beta-sheet at the core. C-PhnF monomers form a dimer by establishing interdomain eight-strand beta-sheets that include core antiparallel and N-terminal two-strand parallel beta-sheets from each monomer. C-PhnF shares strong structural similarity with the chorismate lyase fold, which features a buried active site locked behind two helix-turn-helix loops. The structural comparison of the C-PhnF and UbiC proteins allows us to propose that a similar site in the PhnF structure is adapted for effector binding.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/2fa1_consurf.spt"</scriptWhenChecked>
2FA1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FA1 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16672238 16672238]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fa1 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Edwards, A M.]]
[[Category: Edwards AM]]
[[Category: Gorelik, M.]]
[[Category: Gorelik M]]
[[Category: Lunin, V V.]]
[[Category: Joachimiak A]]
[[Category: MCSG, Midwest Center for Structural Genomics.]]
[[Category: Lunin VV]]
[[Category: Nocek, B P.]]
[[Category: Nocek BP]]
[[Category: Savchenko, A.]]
[[Category: Savchenko A]]
[[Category: Skarina, T.]]
[[Category: Skarina T]]
[[Category: Apc5558]]
[[Category: Effector binding domain]]
[[Category: Mcsg]]
[[Category: Midwest center for structural genomic]]
[[Category: Pnhf]]
[[Category: Protein structure initiative]]
[[Category: Psi]]
[[Category: Regulator]]
[[Category: Transcription]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:38:58 2008''

Latest revision as of 09:22, 14 February 2024

Crystal structure of PhnF C-terminal domain

2fa1, resolution 1.70Å

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