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New page: left|200px<br /> <applet load="2xbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2xbd" /> '''INTERNAL XYLAN BINDING DOMAIN FROM CELLULOM...
 
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[[Image:2xbd.gif|left|200px]]<br />
<applet load="2xbd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2xbd" />
'''INTERNAL XYLAN BINDING DOMAIN FROM CELLULOMONAS FIMI XYLANASE D, NMR, MINIMIZED AVERAGE STRUCTURE'''<br />


==Overview==
==INTERNAL XYLAN BINDING DOMAIN FROM CELLULOMONAS FIMI XYLANASE D, NMR, MINIMIZED AVERAGE STRUCTURE==
BACKGROUND: Many enzymes that digest polysaccharides contain separate, polysaccharide-binding domains. Structures have been previously determined, for a number of cellulose-binding domains (CBDs) from cellulases. RESULTS:, The family IIb xylan-binding domain 1 (XBD1) from Cellulomonas fimi, xylanase D is shown to bind xylan but not cellulose. Its structure is, similar to that of the homologous family IIa CBD from C. fimi Cex, consisting of two four-stranded beta sheets that form a twisted 'beta, sandwich'. The xylan-binding site is a groove made from two tryptophan, residues that stack against the faces of the sugar rings, plus several, hydrogen-bonding polar residues. CONCLUSIONS: The biggest difference, between the family IIa and IIb domains is that in the former the, solvent-exposed ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10425686 (full description)]]
<StructureSection load='2xbd' size='340' side='right'caption='[[2xbd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xbd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XBD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xbd OCA], [https://pdbe.org/2xbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xbd RCSB], [https://www.ebi.ac.uk/pdbsum/2xbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xbd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XYND_CELFI XYND_CELFI] Endo-acting xylanase which displays no detectable activity against polysaccharides other than xylan. Hydrolyzes glucosidic bonds with retention of anomeric configuration.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/2xbd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xbd ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Many enzymes that digest polysaccharides contain separate polysaccharide-binding domains. Structures have been previously determined for a number of cellulose-binding domains (CBDs) from cellulases. RESULTS: The family IIb xylan-binding domain 1 (XBD1) from Cellulomonas fimi xylanase D is shown to bind xylan but not cellulose. Its structure is similar to that of the homologous family IIa CBD from C. fimi Cex, consisting of two four-stranded beta sheets that form a twisted 'beta sandwich'. The xylan-binding site is a groove made from two tryptophan residues that stack against the faces of the sugar rings, plus several hydrogen-bonding polar residues. CONCLUSIONS: The biggest difference between the family IIa and IIb domains is that in the former the solvent-exposed tryptophan sidechains are coplanar, whereas in the latter they are perpendicular, forming a twisted binding site. The binding sites are therefore complementary to the secondary structures of the ligands cellulose and xylan. XBD1 and CexCBD represent a striking example of two proteins that have high sequence similarity but a different function.


==About this Structure==
A family IIb xylan-binding domain has a similar secondary structure to a homologous family IIa cellulose-binding domain but different ligand specificity.,Simpson PJ, Bolam DN, Cooper A, Ciruela A, Hazlewood GP, Gilbert HJ, Williamson MP Structure. 1999 Jul 15;7(7):853-64. PMID:10425686<ref>PMID:10425686</ref>
2XBD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2XBD OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
A family IIb xylan-binding domain has a similar secondary structure to a homologous family IIa cellulose-binding domain but different ligand specificity., Simpson PJ, Bolam DN, Cooper A, Ciruela A, Hazlewood GP, Gilbert HJ, Williamson MP, Structure. 1999 Jul 15;7(7):853-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10425686 10425686]
</div>
<div class="pdbe-citations 2xbd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cellulomonas fimi]]
[[Category: Cellulomonas fimi]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bolam, D.N.]]
[[Category: Bolam DN]]
[[Category: Ciruela, A.]]
[[Category: Ciruela A]]
[[Category: Cooper, A.]]
[[Category: Cooper A]]
[[Category: Gilbert, H.J.]]
[[Category: Gilbert HJ]]
[[Category: Hazlewood, G.P.]]
[[Category: Hazlewood GP]]
[[Category: Simpson, P.J.]]
[[Category: Simpson PJ]]
[[Category: Williamson, M.P.]]
[[Category: Williamson MP]]
[[Category: beta-sheet]]
[[Category: hydrolase]]
[[Category: xylan binding domain]]
[[Category: xylanase]]
 
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Retrieved from "https://proteopedia.org/2xbd"