2jod: Difference between revisions
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New page: left|200px<br /> <applet load="2jod" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jod" /> '''Pac1-Rshort N-terminal EC domain Pacap(6-38... |
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== | ==Pac1-Rshort N-terminal EC domain Pacap(6-38) complex== | ||
The pituitary adenylate cyclase-activating polypeptide (PACAP) receptor is | <StructureSection load='2jod' size='340' side='right'caption='[[2jod]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2jod]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JOD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jod OCA], [https://pdbe.org/2jod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jod RCSB], [https://www.ebi.ac.uk/pdbsum/2jod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jod ProSAT]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/2jod_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jod ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The pituitary adenylate cyclase-activating polypeptide (PACAP) receptor is a class II G protein-coupled receptor that contributes to many different cellular functions including neurotransmission, neuronal survival, and synaptic plasticity. The solution structure of the potent antagonist PACAP (residues 6'-38') complexed to the N-terminal extracellular (EC) domain of the human splice variant hPAC1-R-short (hPAC1-R(S)) was determined by NMR. The PACAP peptide adopts a helical conformation when bound to hPAC1-R(S) with a bend at residue A18' and makes extensive hydrophobic and electrostatic interactions along the exposed beta-sheet and interconnecting loops of the N-terminal EC domain. Mutagenesis data on both the peptide and the receptor delineate the critical interactions between the C terminus of the peptide and the C terminus of the EC domain that define the high affinity and specificity of hormone binding to hPAC1-R(S). These results present a structural basis for hPAC1-R(S) selectivity for PACAP versus the vasoactive intestinal peptide and also differentiate PACAP residues involved in binding to the N-terminal extracellular domain versus other parts of the full-length hPAC1-R(S) receptor. The structural, mutational, and binding data are consistent with a model for peptide binding in which the C terminus of the peptide hormone interacts almost exclusively with the N-terminal EC domain, whereas the central region makes contacts to both the N-terminal and other extracellular parts of the receptor, ultimately positioning the N terminus of the peptide to contact the transmembrane region and result in receptor activation. | |||
Solution structure and mutational analysis of pituitary adenylate cyclase-activating polypeptide binding to the extracellular domain of PAC1-RS.,Sun C, Song D, Davis-Taber RA, Barrett LW, Scott VE, Richardson PL, Pereda-Lopez A, Uchic ME, Solomon LR, Lake MR, Walter KA, Hajduk PJ, Olejniczak ET Proc Natl Acad Sci U S A. 2007 May 8;104(19):7875-80. Epub 2007 Apr 30. PMID:17470806<ref>PMID:17470806</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2jod" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Hormone|Hormone]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Barrett | [[Category: Barrett LW]] | ||
[[Category: Davis-Taber | [[Category: Davis-Taber RA]] | ||
[[Category: Hajduk | [[Category: Hajduk PJ]] | ||
[[Category: Lake | [[Category: Lake MR]] | ||
[[Category: Olejniczak | [[Category: Olejniczak ET]] | ||
[[Category: Pereda-lopez | [[Category: Pereda-lopez A]] | ||
[[Category: Richardson | [[Category: Richardson PL]] | ||
[[Category: Scott | [[Category: Scott VE]] | ||
[[Category: Solomon | [[Category: Solomon LR]] | ||
[[Category: Song | [[Category: Song D]] | ||
[[Category: Sun | [[Category: Sun C]] | ||
[[Category: Uchic | [[Category: Uchic ME]] | ||
[[Category: Walter | [[Category: Walter KA]] | ||
