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[[Image:2gsg.jpg|left|200px]]
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{{STRUCTURE_2gsg|  PDB=2gsg  |  SCENE=  }}
'''Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine'''


==Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine==
<StructureSection load='2gsg' size='340' side='right'caption='[[2gsg]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2gsg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsg OCA], [https://pdbe.org/2gsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gsg RCSB], [https://www.ebi.ac.uk/pdbsum/2gsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gsg ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/2gsg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gsg ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Huntington and related neurological diseases result from expansion of a polyglutamine (polyQ) tract. The linear lattice model for the structure and binding properties of polyQ proposes that both expanded and normal polyQ tracts in the preaggregation state are random-coil structures but that an expanded polyQ repeat contains a larger number of epitopes recognized by antibodies or other proteins. The crystal structure of polyQ bound to MW1, an antibody against polyQ, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities. These results suggest a mechanism for the toxicity of expanded polyQ and a strategy to link anti-polyQ compounds to create high-avidity therapeutics.


==Overview==
The structure of a polyQ-anti-polyQ complex reveals binding according to a linear lattice model.,Li P, Huey-Tubman KE, Gao T, Li X, West AP Jr, Bennett MJ, Bjorkman PJ Nat Struct Mol Biol. 2007 May;14(5):381-7. Epub 2007 Apr 22. PMID:17450152<ref>PMID:17450152</ref>
Huntington and related neurological diseases result from expansion of a polyglutamine (polyQ) tract. The linear lattice model for the structure and binding properties of polyQ proposes that both expanded and normal polyQ tracts in the preaggregation state are random-coil structures but that an expanded polyQ repeat contains a larger number of epitopes recognized by antibodies or other proteins. The crystal structure of polyQ bound to MW1, an antibody against polyQ, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities. These results suggest a mechanism for the toxicity of expanded polyQ and a strategy to link anti-polyQ compounds to create high-avidity therapeutics.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSG OCA].
</div>
<div class="pdbe-citations 2gsg" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
The structure of a polyQ-anti-polyQ complex reveals binding according to a linear lattice model., Li P, Huey-Tubman KE, Gao T, Li X, West AP Jr, Bennett MJ, Bjorkman PJ, Nat Struct Mol Biol. 2007 May;14(5):381-7. Epub 2007 Apr 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17450152 17450152]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
[[Category: Bennett, M J.]]
== References ==
[[Category: Bjorkman, P J.]]
<references/>
[[Category: Huey-Tubman, K E.]]
__TOC__
[[Category: Jr., A P.West.]]
</StructureSection>
[[Category: Li, P.]]
[[Category: Large Structures]]
[[Category: Fv]]
[[Category: Mus musculus]]
[[Category: Monoclonal antibody]]
[[Category: Bennett MJ]]
[[Category: Poly-glutamine]]
[[Category: Bjorkman PJ]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:28:15 2008''
[[Category: Huey-Tubman KE]]
[[Category: Li P]]
[[Category: West Jr AP]]

Latest revision as of 08:04, 30 October 2024

Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine

2gsg, resolution 2.10Å

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