2h6u: Difference between revisions

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[[Image:2h6u.gif|left|200px]]
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{{STRUCTURE_2h6u|  PDB=2h6u  |  SCENE=  }}
'''Crystal structure of 5-hydroxyisourate hydrolase (formerly known as TRP, transthyretin related protein)'''


==Crystal structure of 5-hydroxyisourate hydrolase (formerly known as TRP, transthyretin related protein)==
<StructureSection load='2h6u' size='340' side='right'caption='[[2h6u]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2h6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H6U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6u OCA], [https://pdbe.org/2h6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h6u RCSB], [https://www.ebi.ac.uk/pdbsum/2h6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h6u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HIUH_DANRE HIUH_DANRE] Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/2h6u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h6u ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During early vertebrate evolution, a duplication event in the gene encoding 5-hydroxyisourate hydrolase (HIUase), a widely distributed enzyme of purine metabolism, gave rise to transthyretin (TTR), a thyroid hormone transporter. We report here on the crystal structure of zebra fish HIUase in two different crystal forms. Despite the phylogenetic distance, this structure compares well with those of newly characterized bacterial HIUases, especially with regard to catalytic regions, which are highly preserved. Comparison with TTR structure reveals a highly conserved scaffold, harbouring distinct functional sites located in the same regions of the two vertebrate proteins. Residues that are differentially conserved in HIUases compared to TTR map in putative catalytic regions occupying significant portions of the two halves of a central channel that transverses the whole TTR protein. The evolution of TTR has been accompanied by remarkable changes of the HIUase active sites that gave rise to a channel open at both ends, thus allowing free access to hormone molecules.


==Overview==
Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter.,Zanotti G, Cendron L, Ramazzina I, Folli C, Percudani R, Berni R J Mol Biol. 2006 Oct 13;363(1):1-9. Epub 2006 Aug 2. PMID:16952372<ref>PMID:16952372</ref>
During early vertebrate evolution, a duplication event in the gene encoding 5-hydroxyisourate hydrolase (HIUase), a widely distributed enzyme of purine metabolism, gave rise to transthyretin (TTR), a thyroid hormone transporter. We report here on the crystal structure of zebra fish HIUase in two different crystal forms. Despite the phylogenetic distance, this structure compares well with those of newly characterized bacterial HIUases, especially with regard to catalytic regions, which are highly preserved. Comparison with TTR structure reveals a highly conserved scaffold, harbouring distinct functional sites located in the same regions of the two vertebrate proteins. Residues that are differentially conserved in HIUases compared to TTR map in putative catalytic regions occupying significant portions of the two halves of a central channel that transverses the whole TTR protein. The evolution of TTR has been accompanied by remarkable changes of the HIUase active sites that gave rise to a channel open at both ends, thus allowing free access to hormone molecules.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2H6U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6U OCA].
</div>
<div class="pdbe-citations 2h6u" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter., Zanotti G, Cendron L, Ramazzina I, Folli C, Percudani R, Berni R, J Mol Biol. 2006 Oct 13;363(1):1-9. Epub 2006 Aug 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16952372 16952372]
*[[Transthyretin 3D structures|Transthyretin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Danio rerio]]
[[Category: Danio rerio]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berni, R.]]
[[Category: Berni R]]
[[Category: Cendron, L.]]
[[Category: Cendron L]]
[[Category: Folli, C.]]
[[Category: Folli C]]
[[Category: Percudani, R.]]
[[Category: Percudani R]]
[[Category: Ramazzina, I.]]
[[Category: Ramazzina I]]
[[Category: Zanotti, G.]]
[[Category: Zanotti G]]
[[Category: 5-hydroxyisourate hydrolase]]
[[Category: Allantoin]]
[[Category: Trp]]
[[Category: Uric acid degradation]]
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Retrieved from "https://proteopedia.org/2h6u"