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| [[Image:2h7o.gif|left|200px]]
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| <!--
| | ==Crystal structure of the Rho-GTPase binding domain of YpkA== |
| The line below this paragraph, containing "STRUCTURE_2h7o", creates the "Structure Box" on the page.
| | <StructureSection load='2h7o' size='340' side='right'caption='[[2h7o]], [[Resolution|resolution]] 2.00Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[2h7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H7O FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [https://pdbe.org/2h7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [https://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7o ProSAT]</span></td></tr> |
| {{STRUCTURE_2h7o| PDB=2h7o | SCENE= }}
| | </table> |
| | | == Function == |
| '''Crystal structure of the Rho-GTPase binding domain of YpkA'''
| | [https://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref> |
| | | == References == |
| | | <references/> |
| ==Overview== | | __TOC__ |
| Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| ==About this Structure== | |
| 2H7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA].
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| ==Reference== | |
| Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16959567 16959567]
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| [[Category: Single protein]] | |
| [[Category: Yersinia pseudotuberculosis]] | | [[Category: Yersinia pseudotuberculosis]] |
| [[Category: Bliska, J B.]] | | [[Category: Bliska JB]] |
| [[Category: Ivanov, M.]] | | [[Category: Ivanov M]] |
| [[Category: Prehna, G.]] | | [[Category: Prehna G]] |
| [[Category: Stebbins, C E.]] | | [[Category: Stebbins CE]] |
| [[Category: Gdi]]
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| [[Category: Yersinia]]
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| [[Category: Yopo]]
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| [[Category: Ypka]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:58:03 2008''
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