2z6p: Difference between revisions
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New page: left|200px<br /> <applet load="2z6p" size="450" color="white" frame="true" align="right" spinBox="true" caption="2z6p, resolution 1.80Å" /> '''Crystal Structure o... |
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== | ==Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1== | ||
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central | <StructureSection load='2z6p' size='340' side='right'caption='[[2z6p]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2z6p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6P FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2z6o|2z6o]]</div></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UFC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6p OCA], [https://pdbe.org/2z6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6p RCSB], [https://www.ebi.ac.uk/pdbsum/2z6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6p ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[https://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN]] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z6p_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z6p ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability. | |||
Crystal structure of Ufc1, the Ufm1-conjugating enzyme.,Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256<ref>PMID:17825256</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: | <div class="pdbe-citations 2z6p" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
==See Also== | |||
*[[Ubiquitin-fold modifier|Ubiquitin-fold modifier]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human]] | |||
[[Category: Large Structures]] | |||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
[[Category: Kawakami, T | [[Category: Kawakami, T]] | ||
[[Category: Komatsu, M | [[Category: Komatsu, M]] | ||
[[Category: Kominami, E | [[Category: Kominami, E]] | ||
[[Category: Mizushima ,T | [[Category: Mizushima , T]] | ||
[[Category: Ogasahara, K | [[Category: Ogasahara, K]] | ||
[[Category: Ozaki, Y | [[Category: Ozaki, Y]] | ||
[[Category: Suzuki, A | [[Category: Suzuki, A]] | ||
[[Category: Tanaka, K | [[Category: Tanaka, K]] | ||
[[Category: Tatsumi, K | [[Category: Tatsumi, K]] | ||
[[Category: Yamane, T | [[Category: Yamane, T]] | ||
[[Category: | [[Category: Ligase]] | ||
[[Category: | [[Category: Polymorphism]] | ||
[[Category: | [[Category: Ubiquitin]] | ||
[[Category: | [[Category: Ubl]] | ||
[[Category: | [[Category: Ubl conjugation pathway]] | ||
[[Category: | [[Category: Ufc1]] | ||
[[Category: | [[Category: Ufm1]] | ||
Latest revision as of 10:47, 8 December 2021
Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1
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