2jx5: Difference between revisions

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[[Image:2jx5.jpg|left|200px]]
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{{STRUCTURE_2jx5|  PDB=2jx5  |  SCENE=  }}
'''Solution structure of the ubiquitin domain N-terminal to the S27a ribosomal subunit of Giardia lamblia'''


==Solution structure of the ubiquitin domain N-terminal to the S27a ribosomal subunit of Giardia lamblia==
<StructureSection load='2jx5' size='340' side='right'caption='[[2jx5]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jx5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JX5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jx5 OCA], [https://pdbe.org/2jx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jx5 RCSB], [https://www.ebi.ac.uk/pdbsum/2jx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jx5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8BNY1_GIAIC A8BNY1_GIAIC]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Encoded by a multigene family, ubiquitin is expressed in the form of three precursor proteins, two of which are fusions to the ribosomal subunits S27a and L40. Ubiquitin assists in ribosome biogenesis and also functions as a post-translational modifier after its release from S27a or L40. However, several species do not conserve the ribosomal ubiquitin domains. We report here the solution structure of a distant variant of ubiquitin, found at the N-terminus of S27a in Giardia lamblia, referred to as GlUb(S27a). Despite the considerable evolutionary distance that separates ubiquitin from GlUb(S27a), the structure of GlUb(S27a) is largely identical to that of ubiquitin. The variant domain remains attached to S27a and is part of the assembled holoribosome. Thus, conservation of tertiary structure suggests a role of this variant as a chaperone, while conservation of the primary structure--necessary for ubiquitin's function as a post-translational modifier--is no longer required. Based on these observations, we propose a model to explain the origin of the widespread ubiquitin superfold in eukaryotes.


==Overview==
Sequence and structure evolved separately in a ribosomal ubiquitin variant.,Catic A, Sun ZY, Ratner DM, Misaghi S, Spooner E, Samuelson J, Wagner G, Ploegh HL EMBO J. 2007 Jul 25;26(14):3474-83. Epub 2007 Jun 28. PMID:17599068<ref>PMID:17599068</ref>
Encoded by a multigene family, ubiquitin is expressed in the form of three precursor proteins, two of which are fusions to the ribosomal subunits S27a and L40. Ubiquitin assists in ribosome biogenesis and also functions as a post-translational modifier after its release from S27a or L40. However, several species do not conserve the ribosomal ubiquitin domains. We report here the solution structure of a distant variant of ubiquitin, found at the N-terminus of S27a in Giardia lamblia, referred to as GlUb(S27a). Despite the considerable evolutionary distance that separates ubiquitin from GlUb(S27a), the structure of GlUb(S27a) is largely identical to that of ubiquitin. The variant domain remains attached to S27a and is part of the assembled holoribosome. Thus, conservation of tertiary structure suggests a role of this variant as a chaperone, while conservation of the primary structure--necessary for ubiquitin's function as a post-translational modifier--is no longer required. Based on these observations, we propose a model to explain the origin of the widespread ubiquitin superfold in eukaryotes.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2JX5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_lamblia_atcc_50803 Giardia lamblia atcc 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX5 OCA].
</div>
<div class="pdbe-citations 2jx5" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Sequence and structure evolved separately in a ribosomal ubiquitin variant., Catic A, Sun ZY, Ratner DM, Misaghi S, Spooner E, Samuelson J, Wagner G, Ploegh HL, EMBO J. 2007 Jul 25;26(14):3474-83. Epub 2007 Jun 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17599068 17599068]
*[[Ribosomal protein S27|Ribosomal protein S27]]
[[Category: Giardia lamblia atcc 50803]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Catic, A.]]
__TOC__
[[Category: Misaghi, S.]]
</StructureSection>
[[Category: Ploegh, H L.]]
[[Category: Giardia lamblia ATCC 50803]]
[[Category: Ratner, D M.]]
[[Category: Large Structures]]
[[Category: Samuelson, J.]]
[[Category: Catic A]]
[[Category: Spooner, E.]]
[[Category: Misaghi S]]
[[Category: Sun, Z J.]]
[[Category: Ploegh HL]]
[[Category: Wagner, G.]]
[[Category: Ratner DM]]
[[Category: Evolution]]
[[Category: Samuelson J]]
[[Category: Glub]]
[[Category: Spooner E]]
[[Category: Recombination]]
[[Category: Sun ZJ]]
[[Category: Ribosomal protein]]
[[Category: Wagner G]]
[[Category: Ribosome]]
[[Category: Ubiquitin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:22:27 2008''

Latest revision as of 12:49, 20 December 2023

Solution structure of the ubiquitin domain N-terminal to the S27a ribosomal subunit of Giardia lamblia

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