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[[Image:2npr.gif|left|200px]]


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==Structural Studies on Plasmodium vivax Merozoite Surface Protein-1==
The line below this paragraph, containing "STRUCTURE_2npr", creates the "Structure Box" on the page.
<StructureSection load='2npr' size='340' side='right'caption='[[2npr]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2npr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax_strain_Belem Plasmodium vivax strain Belem]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NPR FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2npr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npr OCA], [https://pdbe.org/2npr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2npr RCSB], [https://www.ebi.ac.uk/pdbsum/2npr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2npr ProSAT]</span></td></tr>
{{STRUCTURE_2npr|  PDB=2npr  |  SCENE=  }}
</table>
 
== Function ==
'''Structural Studies on Plasmodium vivax Merozoite Surface Protein-1'''
[https://www.uniprot.org/uniprot/Q8I1L1_PLAVI Q8I1L1_PLAVI]
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2npr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npr ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.
Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.


==About this Structure==
Structural studies on Plasmodium vivax merozoite surface protein-1.,Babon JJ, Morgan WD, Kelly G, Eccleston JF, Feeney J, Holder AA Mol Biochem Parasitol. 2007 May;153(1):31-40. Epub 2007 Jan 30. PMID:17343930<ref>PMID:17343930</ref>
2NPR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPR OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural studies on Plasmodium vivax merozoite surface protein-1., Babon JJ, Morgan WD, Kelly G, Eccleston JF, Feeney J, Holder AA, Mol Biochem Parasitol. 2007 May;153(1):31-40. Epub 2007 Jan 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17343930 17343930]
</div>
[[Category: Plasmodium vivax]]
<div class="pdbe-citations 2npr" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Babon, J J.]]
<references/>
[[Category: Eccleston, J F.]]
__TOC__
[[Category: Feeney, J.]]
</StructureSection>
[[Category: Holder, A A.]]
[[Category: Large Structures]]
[[Category: Kelly, G.]]
[[Category: Plasmodium vivax strain Belem]]
[[Category: Morgan, W D.]]
[[Category: Babon JJ]]
[[Category: Egf-like domain]]
[[Category: Eccleston JF]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:45:16 2008''
[[Category: Feeney J]]
[[Category: Holder AA]]
[[Category: Kelly G]]
[[Category: Morgan WD]]

Latest revision as of 00:09, 28 December 2023

Structural Studies on Plasmodium vivax Merozoite Surface Protein-1

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