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[[Image:2ns1.jpg|left|200px]]
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{{STRUCTURE_2ns1|  PDB=2ns1  |  SCENE=  }}
'''Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK'''


==Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK==
<StructureSection load='2ns1' size='340' side='right'caption='[[2ns1]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ns1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.962&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns1 OCA], [https://pdbe.org/2ns1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ns1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.


==Overview==
Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A.,Gruswitz F, O'Connell J 3rd, Stroud RM Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:17190799<ref>PMID:17190799</ref>
Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2NS1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS1 OCA].
</div>
<div class="pdbe-citations 2ns1" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A., Gruswitz F, O'Connell J 3rd, Stroud RM, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):42-7. Epub 2006 Dec 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17190799 17190799]
*[[Ion channels 3D structures|Ion channels 3D structures]]
[[Category: Escherichia coli]]
== References ==
[[Category: Protein complex]]
<references/>
[[Category: CSMP, Center for Structures of Membrane Proteins.]]
__TOC__
[[Category: Gruswitz, F.]]
</StructureSection>
[[Category: III, J O.Connell.]]
[[Category: Escherichia coli K-12]]
[[Category: Stroud, R M.]]
[[Category: Large Structures]]
[[Category: Adp]]
[[Category: Gruswitz F]]
[[Category: Ammonia]]
[[Category: O'Connell III J]]
[[Category: Bog]]
[[Category: Stroud RM]]
[[Category: Center for structures of membrane protein]]
[[Category: Channel]]
[[Category: Csmp]]
[[Category: Inhibitor]]
[[Category: Membrane protein]]
[[Category: Protein structure initiative]]
[[Category: Protein-protein complex]]
[[Category: Psi-2]]
[[Category: Regulatory]]
[[Category: Signal protein]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:50:27 2008''

Latest revision as of 10:19, 30 August 2023

Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK

2ns1, resolution 1.96Å

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