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[[Image:2ns2.gif|left|200px]]
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{{STRUCTURE_2ns2|  PDB=2ns2  |  SCENE=  }}
'''Crystal Structure of Spindlin1'''


==Crystal Structure of Spindlin1==
<StructureSection load='2ns2' size='340' side='right'caption='[[2ns2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ns2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns2 OCA], [https://pdbe.org/2ns2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPIN1_HUMAN SPIN1_HUMAN] May play a role in cell-cycle regulation during the transition from gamete to embryo (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ns2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Spindlin1, a meiotic spindle-binding protein that is highly expressed in ovarian cancer cells, was first identified as a gene involved in gametogenesis. It appeared to be a target for cell cycle-dependent phosphorylation and was demonstrated to disturb the cell cycle. Here we report the crystal structure of human spindlin1 to 2.2A of resolution, representing the first three-dimensional structure from the spin/ssty (Y-linked spermiogenesis-specific transcript) gene family. The refined structure, containing three repeats of five/four anti-parallel beta-strands, exhibits a novel arrangement of tandem Tudor-like domains. Two phosphate ions, chelated by Thr-95 and other residues, appear to stabilize the long loop between domains I and II, which might mediate the cell cycle regulation activity of spindlin1. Flow cytometry experiments indicate that cells expressing spindlin1 display a different cell cycle distribution in mitosis, whereas those expressing a T95A mutant, which had a great decrease in phosphorous content, have little effect on the cell cycle. We further identified associations of spindlin1 with nucleic acid to provide a biochemical basis for its cell cycle regulation and other functions.


==Overview==
Structure of human spindlin1. Tandem tudor-like domains for cell cycle regulation.,Zhao Q, Qin L, Jiang F, Wu B, Yue W, Xu F, Rong Z, Yuan H, Xie X, Gao Y, Bai C, Bartlam M, Pei X, Rao Z J Biol Chem. 2007 Jan 5;282(1):647-56. Epub 2006 Nov 1. PMID:17082182<ref>PMID:17082182</ref>
Spindlin1, a meiotic spindle-binding protein that is highly expressed in ovarian cancer cells, was first identified as a gene involved in gametogenesis. It appeared to be a target for cell cycle-dependent phosphorylation and was demonstrated to disturb the cell cycle. Here we report the crystal structure of human spindlin1 to 2.2A of resolution, representing the first three-dimensional structure from the spin/ssty (Y-linked spermiogenesis-specific transcript) gene family. The refined structure, containing three repeats of five/four anti-parallel beta-strands, exhibits a novel arrangement of tandem Tudor-like domains. Two phosphate ions, chelated by Thr-95 and other residues, appear to stabilize the long loop between domains I and II, which might mediate the cell cycle regulation activity of spindlin1. Flow cytometry experiments indicate that cells expressing spindlin1 display a different cell cycle distribution in mitosis, whereas those expressing a T95A mutant, which had a great decrease in phosphorous content, have little effect on the cell cycle. We further identified associations of spindlin1 with nucleic acid to provide a biochemical basis for its cell cycle regulation and other functions.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2NS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS2 OCA].
</div>
<div class="pdbe-citations 2ns2" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of human spindlin1. Tandem tudor-like domains for cell cycle regulation., Zhao Q, Qin L, Jiang F, Wu B, Yue W, Xu F, Rong Z, Yuan H, Xie X, Gao Y, Bai C, Bartlam M, Pei X, Rao Z, J Biol Chem. 2007 Jan 5;282(1):647-56. Epub 2006 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17082182 17082182]
*[[Spindlin|Spindlin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bai, C.]]
[[Category: Bai C]]
[[Category: Bartlam, M.]]
[[Category: Bartlam M]]
[[Category: Gao, Y.]]
[[Category: Gao Y]]
[[Category: Jiang, F.]]
[[Category: Jiang F]]
[[Category: Qin, L.]]
[[Category: Qin L]]
[[Category: Rong, Z.]]
[[Category: Rong Z]]
[[Category: Wu, B.]]
[[Category: Wu B]]
[[Category: Xie, X.]]
[[Category: Xie X]]
[[Category: Xu, F.]]
[[Category: Xu F]]
[[Category: Yuan, H.]]
[[Category: Yuan H]]
[[Category: Yue, W.]]
[[Category: Yue W]]
[[Category: Zhao, Q.]]
[[Category: Zhao Q]]
[[Category: Beta barrel]]
[[Category: Repeat domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:50:27 2008''
Retrieved from "https://proteopedia.org/2ns2"