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[[Image:2ns7.jpg|left|200px]]
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{{STRUCTURE_2ns7|  PDB=2ns7  |  SCENE=  }}
'''How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor'''


==How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor==
<StructureSection load='2ns7' size='340' side='right'caption='[[2ns7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ns7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns7 OCA], [https://pdbe.org/2ns7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ns7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A 16-residue peptide, called Tip, induces the tetracycline repressor TetR as efficiently as the antibiotic tetracycline when fused to the N or C terminus of another protein. This is unusual because the majority of in vitro selected peptides, such as Tip, inhibit protein function, and agonist peptides are only rarely identified. We elucidated the atomic mechanism of TetR induction by Tip from crystal structures of TetR in complex with Tip and of free TetR. Peptide induction ultimately results in the same movements of DNA reading heads, but Tip accomplishes this by very different molecular interactions from tetracycline involving important contacts to the TetR surface. As a direct consequence, an alternate pathway of allostery becomes possible that makes ample use of intersubunit interactions. For the first time it is possible to show in atomic detail how a small molecule controlled bacterial transcription factor such as TetR becomes responsive to protein-protein interactions, characteristic of gene transcription regulation in higher organisms.


==Overview==
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.,Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:17374541<ref>PMID:17374541</ref>
A 16-residue peptide, called Tip, induces the tetracycline repressor TetR as efficiently as the antibiotic tetracycline when fused to the N or C terminus of another protein. This is unusual because the majority of in vitro selected peptides, such as Tip, inhibit protein function, and agonist peptides are only rarely identified. We elucidated the atomic mechanism of TetR induction by Tip from crystal structures of TetR in complex with Tip and of free TetR. Peptide induction ultimately results in the same movements of DNA reading heads, but Tip accomplishes this by very different molecular interactions from tetracycline involving important contacts to the TetR surface. As a direct consequence, an alternate pathway of allostery becomes possible that makes ample use of intersubunit interactions. For the first time it is possible to show in atomic detail how a small molecule controlled bacterial transcription factor such as TetR becomes responsive to protein-protein interactions, characteristic of gene transcription regulation in higher organisms.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2NS7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS7 OCA].
</div>
<div class="pdbe-citations 2ns7" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor., Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA, J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17374541 17374541]
*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
[[Category: Escherichia coli]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Berens, C.]]
__TOC__
[[Category: Hillen, W.]]
</StructureSection>
[[Category: Klotzsche, M.]]
[[Category: Escherichia coli K-12]]
[[Category: Luckner, S R.]]
[[Category: Large Structures]]
[[Category: Muller, Y A.]]
[[Category: Berens C]]
[[Category: Helix-turn-helix]]
[[Category: Hillen W]]
[[Category: Transcription regulator]]
[[Category: Klotzsche M]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:50:57 2008''
[[Category: Luckner SR]]
[[Category: Muller YA]]

Latest revision as of 08:55, 25 October 2023

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

2ns7, resolution 2.40Å

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